Rack for sample tubes and reagent holders

• US 10,179,910 B2
• Filed: 02/08/2016
• Issued: 01/15/2019
• Est. Priority Date: 07/13/2007
• Status: Active Grant

• Current Assignee: HandyLab Incorporated (Becton, Dickinson & Co)
• Original Assignee: HandyLab Incorporated (Becton, Dickinson & Co)
• Inventors: Duffy, Patrick, Wilson, Kerry, Handique, Kalyan, Williams, Jeff
• Primary Examiner(s): Wallenhorst, Maureen
• Application Number:
  US15/017,977
• Publication Number:
  US 20160333337A1
• Time in Patent Office: 1,072 Days
• Field of Search: 436 43, 436 47, 436 94, 436147, 436174, 436177, 436178, 436180, 422 63, 422 65, 422 681, 422509, 422527, 422560, 422561, 422562, 422565, 4352871, 4352872
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 3/5082 Test tubes per se
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 9/06 Test-tube stands; Test-tube holders
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: B03C 1/01 by addition of magnetic adjuvants
• CPC Class Codes: B03C 1/0332 using permanent magnets
• CPC Class Codes: B03C 1/288 disposed at the outer circumference of a recipient
• CPC Class Codes: B03C 1/30 Combinations with other devices, not otherwise provided for
• CPC Class Codes: B03C 2201/18 Magnetic separation whereby the particles are suspended in a liquid
• CPC Class Codes: B03C 2201/26 for use in medical applications
• CPC Class Codes: C12N 15/1003 Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
• CPC Class Codes: F16K 2099/0084 Chemistry or biology, e.g. "lab-on-a-chip" technology
• CPC Class Codes: F16K 99/0001 Microvalves microdevices B81B1/00; manufacture or treatment of devices or systems in or on a substrate B81C1/00; microfluidic structures B01L3/5027; micropumps F04B19/006
• CPC Class Codes: F16K 99/003 Valves for single use only
• CPC Class Codes: F16K 99/0032 using phase transition or influencing viscosity
• CPC Class Codes: F16K 99/0044 using thermo-electric means
• CPC Class Codes: F16K 99/0061 actuated by an expanding gas or liquid volume
• CPC Class Codes: G01N 2035/00772 mechanical or optical code other than bar code
• CPC Class Codes: G01N 2035/00801 Holders for sample carriers, e.g. trays, caroussel, racks
• CPC Class Codes: G01N 2035/00881 : network configurations
• CPC Class Codes: G01N 2035/0493 : Locating samples; identifying different tube sizes
• CPC Class Codes: G01N 35/0099 : comprising robots or similar manipulators robots per se B25J
• CPC Class Codes: G01N 35/026 : having blocks or racks of reaction cells or cuvettes
• CPC Class Codes: G01N 35/1002 : Reagent dispensers
• CPC Class Codes: Y10T 436/113332 : with conveyance of sample along a test line in a container or rack

Abstract

A rack for holding samples and various reagents, wherein the rack may be used for loading the samples and reagents prior to using the reagents. The rack accepts complementary reagent holders, each of which contain a set of reagents for carrying out a predetermined processing operation, such as preparing biological samples for amplifying and detecting polynucleotides extracted from the samples.

Claims

1. A method of preparing a plurality of samples for extraction of nucleic acid contained in the samples using a housing comprising a top surface comprising a plurality of slots arranged parallel to an axis between a first side and a second side of the top surface, each of the plurality of slots defining an opening in the first side of the top surface and extending toward the second side of the top surface, the housing further comprising a first sample tube holder and a second sample tube holder, the method comprising:

sliding a first reagent holder through the opening of a first slot of the plurality of slots;

inserting a first sample tube containing a first sample into the first sample tube holder arranged in line with the first slot;

sliding a second reagent holder through the opening of a second slot of the plurality of slots;

inserting a second sample tube containing the second sample into a second sample tube holder arranged in line with the second slot;

inserting the housing into a receiving bay of a diagnostic apparatus configured to extract nucleic acid from the first sample and the second sample independently; and

applying heat to a portion of the first reagent holder when the first reagent holder is inserted into the first slot of the housing and when the housing is located in the receiving bay of the diagnostic apparatus to allow for extraction of the nucleic acid.

2. The method of claim 1, wherein the first reagent holder and the second reagent holder each comprise a process container and one or more reagent receptacles containing reagents.

3. The method of claim 2, further comprising dispensing the first sample into one or more reagent receptacles of the first reagent holder.

4. The method of claim 2, further comprising inserting the housing into a heater unit of the diagnostic apparatus configured to independently heat the process containers of the first reagent holder and the second reagent holder.

5. The method of claim 1, wherein inserting the housing into the receiving bay further comprises inserting the housing such that an interior of the housing around the process containers of the first reagent holder and the second reagent holder has clearance for a heater assembly.

6. The method of claim 1, wherein inserting the housing into the receiving bay of the diagnostic apparatus comprises inserting a first process container of the first reagent holder and a second process container of the second reagent holder into a heater assembly and magnetic separator, and wherein the method further comprises heating, using the heater assembly, the first process container of the first reagent holder independently of heating the second process container of the second reagent holder.

7. The method of claim 2, wherein the method further comprises separating, using the magnetic separator, liquid materials in the first process container of the first reagent holder independently of separating liquid materials in the second process container of the second reagent holder.

8. The method of claim 1, wherein inserting the housing into the receiving bay further comprises placing the housing in the receiving bay in a single orientation.

9. The method of claim 1, wherein inserting the first sample tube containing the first sample into the first sample tube holder and inserting the second sample tube containing the second sample into the second sample tube holder further comprises inserting the first sample tube and the second sample tube into a dedicated region of the housing.

10. A method of preparing a plurality of samples for extraction of nucleic acid contained in the samples, the method comprising:

inserting a first reagent holder into an opening of a first slot of a plurality of slots of a housing comprising a first sample tube holder and a second sample tube holder;

inserting a first sample tube containing a first sample into the first sample tube holder aligned with the first slot;

inserting a second reagent holder into an opening of a second slot of the plurality of slots of the housing;

inserting a second sample tube containing a second sample into the second sample tube holder aligned with the second slot;

inserting the housing into a receiving bay of a diagnostic apparatus configured to extract nucleic acid from the first sample and the second sample independently; and

applying heat to a portion of the first reagent holder when the first reagent holder is inserted into the first slot of the housing and when the housing is located in the receiving bay of the diagnostic apparatus to facilitate extraction of the nucleic acid.

11. The method of claim 10, wherein the first reagent holder and the second reagent holder each comprise a process container and one or more reagent receptacles containing reagents.

12. The method of claim 11, further comprising dispensing the first sample into one or more reagent receptacles of the first reagent holder.

13. The method of claim 11, further comprising inserting the housing into a heater unit of the diagnostic apparatus configured to independently heat the process containers of the first reagent holder and the second reagent holder.

14. The method of claim 10, wherein inserting the housing into the receiving bay further comprises inserting the housing such that an interior of the housing around the process containers of the first reagent holder and the second reagent holder has clearance for a heater assembly.

15. The method of claim 10, wherein inserting the housing into the receiving bay of the diagnostic apparatus comprises inserting a first process container of the first reagent holder and a second process container of the second reagent holder into a heater assembly and magnetic separator, and wherein the method further comprises heating, using the heater assembly, the first process container of the first reagent holder independently of heating the second process container of the second reagent holder.

16. The method of claim 11, wherein the method further comprises separating, using the magnetic separator, liquid materials in the first process container of the first reagent holder independently of separating liquid materials in the second process container of the second reagent holder.

17. The method of claim 10, wherein inserting the housing into the receiving bay further comprises placing the housing in the receiving bay in a single orientation.

18. The method of claim 10, wherein inserting the first sample tube containing the first sample into the first sample tube holder and inserting the second sample tube containing the second sample into the second sample tube holder further comprises inserting the first sample tube and the second sample tube into a dedicated region of the housing.