Apparatus and method for separating and concentrating fluids containing multiple components
First Claim
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1. A method of separating a component of a multiple component material, comprising:
- separating, within a container system, at least a portion of a solid portion from a liquid portion of a multicomponent material at least by retaining the portion of the solid portion of the multiple component material in a first section of the container system with a filtration system having a first filter media portion positioned between the first section and a second section of the container system, wherein a buoy is slidably received within the second section, the buoy having a first buoy member and a second buoy member;
forming at least a first fraction and a second fraction of the liquid portion after the liquid portion has moved from the first section to the second section, wherein the buoy moves within the second section to isolate the first fraction at least from the second fraction;
removing the first section from the second section following fractionation such that the first filter media portion is removed from the second section, wherein the first filter media portion retains the portion of the solid portion of the multiple component material in the first section during fractionation; and
extracting the first fraction from the second section.
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Abstract
An apparatus may allow separating and collecting a fraction of a sample. The apparatus, when used with a centrifuge, allows for the creation of at least three fractions in the apparatus. It also provides for a new method of extracting the buffy coat phase from a whole blood sample and mesenchymal stem cells from bone reaming material. A buoy system that may include a first buoy portion and a second buoy member operably interconnected may be used to form at least three fractions from a sample during a substantially single centrifugation process. Therefore, the separation of various fractions may be substantially quick and efficient.
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Citations
23 Claims
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1. A method of separating a component of a multiple component material, comprising:
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separating, within a container system, at least a portion of a solid portion from a liquid portion of a multicomponent material at least by retaining the portion of the solid portion of the multiple component material in a first section of the container system with a filtration system having a first filter media portion positioned between the first section and a second section of the container system, wherein a buoy is slidably received within the second section, the buoy having a first buoy member and a second buoy member; forming at least a first fraction and a second fraction of the liquid portion after the liquid portion has moved from the first section to the second section, wherein the buoy moves within the second section to isolate the first fraction at least from the second fraction; removing the first section from the second section following fractionation such that the first filter media portion is removed from the second section, wherein the first filter media portion retains the portion of the solid portion of the multiple component material in the first section during fractionation; and extracting the first fraction from the second section. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method of separating a component of a multiple component material, comprising:
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placing a volume of the multiple component material, including a liquid portion and a solid portion, in a removable filter section of a container, the container having at least the filter section and a fractionation section, wherein a buoy is slidably received within the fractionation section, the buoy having a first buoy member and a second buoy member; separating the solid portion from the liquid portion at least by retaining at least a portion of the solid portion of the multiple component material in the filter section of the container; forming a fraction of the liquid portion in the fractionation section of the container, wherein the buoy moves within the fractionation section to isolate the fraction of the liquid portion; maintaining a connection of the filter section and the fractionation section while forming the fraction of the liquid portion in the fractionation section of the container, wherein the filter section retains the portion of the solid portion of the multiple component material; and extracting the fraction from the fractionation section of the container. - View Dependent Claims (12, 13, 14, 15, 16, 17)
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18. A method of separating a component of a multiple component material, comprising:
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providing a container system for separating at least a first portion of a solid portion from a liquid portion of a multiple component material at least by retaining the first portion of the solid portion of the multiple component material in a first section of the container system with a filtration system having a first filter media portion positioned between the first section and a second section of the container system; after the liquid portion has moved from the first section to the second section, providing a fractionation system to form at least one fraction of the liquid portion between a first buoy member and a second buoy member, wherein the first filter media portion retains the first portion of the solid portion of the multiple component material during forming of the at least one fraction of the liquid portion; and providing access within the second section to extract the at least one fraction of the liquid portion from the second section from between the first buoy member and the second buoy member; wherein the first section is removably coupled to the second section. - View Dependent Claims (19, 20, 21, 22, 23)
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Specification