Capacitance detection in a droplet actuator

• US 10,183,292 B2
• Filed: 12/14/2015
• Issued: 01/22/2019
• Est. Priority Date: 02/15/2007
• Status: Active Grant

• Current Assignee: Advanced Liquid Logic, Inc. (Illumina Incorporated)
• Original Assignee: Advanced Liquid Logic, Inc. (Illumina Incorporated)
• Inventors: Sturmer, Ryan A., Pollack, Michael G., Pamula, Vamsee K., Srinivasan, Vijay, Paik, Philip Y.
• Primary Examiner(s): Hoque, Farhana
• Application Number:
  US14/968,094
• Publication Number:
  US 20160096174A1
• Time in Patent Office: 1,135 Days
• Field of Search: 205 98, 422 67, 324664
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2300/0627 Sensor or part of a sensor is integrated
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0809 rectangular shaped
• CPC Class Codes: B01L 2400/0427 Electrowetting
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502784 specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08
• CPC Class Codes: B01L 3/502792 for moving individual droplets on a plate, e.g. by locally altering surface tension
• CPC Class Codes: G01N 2203/00 Investigating strength properties of solid materials by application of mechanical stress
• CPC Class Codes: G01N 27/22 by investigating capacitance
• CPC Class Codes: G01N 27/223 for determining moisture content, e.g. humidity rain detectors on vehicle windows B60S1/0825
• CPC Class Codes: G01N 27/227 Sensors changing capacitance upon adsorption or absorption of fluid components, e.g. electrolyte-insulator-semiconductor sensors, MOS capacitors G01N27/225 takes precedence
• CPC Class Codes: G01V 3/02 operating with propagation of electric current

Abstract

A method, circuit and apparatus for detecting capacitance on a droplet actuator, inter alia, for determining the presence, partial presence or absence of a droplet at an electrode on a droplet actuator by: (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged on the substrate for conducting droplet operations on a surface of the substrate; (ii) a capacitance detection circuit for detecting capacitance at the droplet operations surface at one or more of the electrodes; (b) detecting capacitance at the droplet operations surface at one or more of the electrodes; and (c) determining from the capacitance the presence, partial presence or absence of a droplet at the droplet operations surface at the electrode.

Claims

1. A droplet actuator for manipulating a fluid using an electrical field, comprising:

(a) an actuator electrode configured to communicate the electrical field to the fluid;

(b) a reference electrode in communication with the actuator electrode and in selective communication with at least one of a ground node, a high voltage node and a high impedance node having an intermediate potential;

(c) a capacitance detection circuit in communication with the reference electrode and configured to detect a capacitance of the fluid; and

(d) a processor in communication with the capacitor detection circuit and configured to determine a position of the fluid based on the detected capacitance of the fluid.

2. The droplet actuator of claim 1 wherein the actuator electrode is configured for mediating droplet operations by a means comprising electrowetting.

3. The droplet actuator of claim 1 wherein the actuator electrode is configured for mediating droplet operations by a means not comprising dielectrophoresis.

4. The droplet actuator of claim 1 further comprising a switch configured to selectively enable communication between the actuator and reference electrodes.

5. The droplet actuator of claim 1 further comprising a plurality of actuator nodes in communication with the reference electrode.

6. The droplet actuator of claim 5 wherein the plurality of actuator nodes sequentially communicate with the reference electrode.

7. The droplet actuator of claim 1 further comprising a voltage source in communication with the actuator electrode.

8. The droplet actuator of claim 7 further comprising a switch configured to enable selective communication between the voltage source and the actuator electrode.

9. The droplet actuator of claim 1 wherein the reference electrode further comprises another actuator electrode.

10. The droplet actuator of claim 1 further comprising a switch configured to selectively enable communication between the actuator electrode and at least one of the ground, high voltage and high impedance nodes.

11. The droplet actuator of claim 1 wherein the processor is further configured to conduct droplet processes while the actuator electrode is in communication with the ground node.

12. The droplet actuator of claim 1 wherein the processor is further configured to conduct droplet detection processes while the actuator electrode is in communication with the high impedance node.

13. The droplet actuator of claim 1 wherein the processor is further configured to enable communication between the actuator electrode and the voltage source while the actuator electrode is in communication with the high impedance node.

14. The droplet actuator of claim 1 wherein the capacitance detection circuit further comprises a protection circuit configured to regulate a voltage entering the capacitance detection circuit to minimize damage caused by a high voltage condition.

15. The droplet actuator of claim 14 wherein the capacitance detection circuit comprises at least one of a voltage divider and a capacitor.

16. The droplet actuator of claim 1 wherein the capacitance detection circuit comprises at least one of an operational amplifier, an integrating amplifier and an analog-to-digital converter.

17. The droplet actuator of claim 1 wherein the processor is further configured to determine that the fluid is not present at the actuator electrode in response to receiving an electrical value associated with the detected capacitance.

18. The droplet actuator of claim 1 wherein the processor is further configured to determine that the fluid is present at the actuator electrode in response to receiving an electrical value associated with the detected capacitance.

19. The droplet actuator of claim 1 wherein the fluid comprises a droplet.

20. The droplet actuator of claim 1 wherein the fluid comprises a droplet comprising beads.

21. The droplet actuator of claim 1 wherein the fluid comprises a droplet comprising cells.

22. The droplet actuator of claim 1 wherein the fluid comprises a filler fluid.

23. The droplet actuator of claim 1 wherein the fluid comprises a filler fluid comprising an oil.

24. The droplet actuator of claim 1 further comprising first and second substrates separated by the fluid, wherein the first substrate comprises the actuator electrode and the second substrate comprises the reference electrode.

25. The droplet actuator of claim 24 wherein the fluid comprises a droplet and the droplet is located over the actuator electrode.

26. The droplet actuator of claim 25 wherein the processor is further configured to initiate a measurement of an amount by which a portion of the droplet overlaps the actuator electrode.

27. The droplet actuator of claim 24 wherein the first substrate comprises a plurality of actuator electrodes and each actuator electrode is equidistant from the reference electrode.

28. The droplet actuator of claim 27 wherein the first substrate comprises a grid or array of actuator electrodes and the second substrate comprises a single reference electrode that is substantially equidistant from each of the actuator electrodes, wherein the measurement at each of the actuator electrodes in the grid or array may be determined based on the single reference electrode.

29. The droplet actuator of claim 27 wherein the first substrate comprises a grid or array of actuator electrodes and the detected capacitance measurement is based upon two of more of the actuator electrodes.