Compositions and methods for detecting mecC-containing methicillin-resistant Staphylococcus aureus

• US 10,190,178 B2
• Filed: 01/08/2018
• Issued: 01/29/2019
• Est. Priority Date: 12/02/2014
• Status: Active Grant

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: Roche Molecular Systems (Roche Holding AG)
• Inventors: Johnson, Jenny A., Hayes, Ashley
• Primary Examiner(s): Bertagna, Angela M.
• Application Number:
  US15/864,500
• Publication Number:
  US 20180148771A1
• Time in Patent Office: 386 Days
• Field of Search: None
• CPC Class Codes: C12Q 1/689 for bacteria
• CPC Class Codes: C12Q 2600/112 Disease subtyping, staging or classification
• CPC Class Codes: C12Q 2600/158 Expression markers
• CPC Class Codes: C12Q 2600/16 Primer sets for multiplex assays

Abstract

Methods for the rapid detection of the presence or absence of mecC-containing Staphylococcus aureus (mecC-MRSA) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the genes for mecC-MRSA, along with kits are provided that are designed for the detection of mecC-MRSA.

Claims

1. A kit for detecting a nucleic acid of mecC-containing methicillin-resistant Staphylococcus aureus (mecC-MRSA) comprising:

a first oligonucleotide consisting of a sequence selected from the group consisting of SEQ ID NOs;

1, 2, 6, and 8;

a second oligonucleotide configured to hybridize to a portion of an orfX gene, consisting of SEQ ID NO;

9; and

a detectably labeled third oligonucleotide configured to hybridize to an amplicon generated by the first oligonucleotide and the second oligonucleotide, consisting of SEQ ID NO;

10, or a complement thereof.

2. The kit of claim 1, wherein the detectably labeled third oligonucleotide comprises a donor fluorescent moiety and a corresponding acceptor fluorescent moiety.

3. The kit of claim 2, wherein the acceptor fluorescent moiety is a quencher.

4. The kit of claim 1, further comprising nucleoside triphosphates, nucleic acid polymerase, and buffers necessary for the function of the nucleic acid polymerase.