Generating a fluid stream in a microfluidic device
First Claim
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1. A method of operating a flow cytometer, comprising:
- providing a variable volume container having a flexible wall;
establishing an amount of fluid in said variable volume container;
exerting an amount of pressure with an amount of a gas on an exterior surface of said flexible wall of said variable volume container, wherein said flexible wall comprises at least two flexible layers, a first flexible layer compatible with said amount of gas and a second flexible layer compatible with said amount of fluid;
collecting said amount of gas between said first flexible layer and said second flexible layer;
providing a conduit between said variable volume container and a flow path of said flow cytometer;
generating a fluid stream of said amount of fluid in said flow path of said flow cytometer by exerting said amount of pressure with said amount of gas on said exterior surface of said flexible wall of said variable volume container;
generating a fluid pressure of said fluid stream of said amount of fluid inside of said flow cytometer within the range of about 25 psi and about 50 psi;
regulating said fluid stream within said flow path of said flow cytometer having said fluid pressure within the range of about 25 psi and about 50 psi for analysis of a plurality of particles entrained within said fluid stream;
intermittently entraining one of said plurality of particles in said fluid stream in said flow path of said flow cytometer; and
analyzing said plurality of particles entrained within said fluid stream.
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Abstract
A fluid handling and delivery system useful in generating a fluid stream in the flow path of microfluidic device.
34 Citations
15 Claims
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1. A method of operating a flow cytometer, comprising:
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providing a variable volume container having a flexible wall; establishing an amount of fluid in said variable volume container; exerting an amount of pressure with an amount of a gas on an exterior surface of said flexible wall of said variable volume container, wherein said flexible wall comprises at least two flexible layers, a first flexible layer compatible with said amount of gas and a second flexible layer compatible with said amount of fluid; collecting said amount of gas between said first flexible layer and said second flexible layer; providing a conduit between said variable volume container and a flow path of said flow cytometer; generating a fluid stream of said amount of fluid in said flow path of said flow cytometer by exerting said amount of pressure with said amount of gas on said exterior surface of said flexible wall of said variable volume container; generating a fluid pressure of said fluid stream of said amount of fluid inside of said flow cytometer within the range of about 25 psi and about 50 psi; regulating said fluid stream within said flow path of said flow cytometer having said fluid pressure within the range of about 25 psi and about 50 psi for analysis of a plurality of particles entrained within said fluid stream; intermittently entraining one of said plurality of particles in said fluid stream in said flow path of said flow cytometer; and analyzing said plurality of particles entrained within said fluid stream. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification