Cell capture system and method of use
First Claim
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1. A system for isolating and analyzing a set of particles from a biological sample, comprising:
- a substrate defining a set of chambers defined at a broad surface, wherein each chamber in the set of chambers is configured to retain a single particle of the set of particles and comprises;
a set of walls defining a chamber volume, a chamber length, and a chamber cross-section;
an open surface permitting access of the single particle to the chamber volume from a direction perpendicular to the broad surface of the substrate; and
wherein each chamber of the set of chambers is fluidly isolated from an adjacent chamber by the set of walls of each chamber, and wherein fluid received at the inlet channel enters each chamber only by way of the open surface of each chamber;
an inlet channel positioned superior the set of chambers and directly fluidly coupled to the set of chambers, whereby fluid received at the inlet channel concurrently accesses each of the chambers in the set of chambers at the open surface of each chamber; and
a fluid delivery module fluidly coupled to the inlet channel operable and configured to distribute a reagent fluid in a direction substantially parallel to the broad surface through the inlet channel, permitting access of the reagent fluid to the set of chambers.
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Abstract
A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
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Citations
19 Claims
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1. A system for isolating and analyzing a set of particles from a biological sample, comprising:
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a substrate defining a set of chambers defined at a broad surface, wherein each chamber in the set of chambers is configured to retain a single particle of the set of particles and comprises; a set of walls defining a chamber volume, a chamber length, and a chamber cross-section; an open surface permitting access of the single particle to the chamber volume from a direction perpendicular to the broad surface of the substrate; and wherein each chamber of the set of chambers is fluidly isolated from an adjacent chamber by the set of walls of each chamber, and wherein fluid received at the inlet channel enters each chamber only by way of the open surface of each chamber; an inlet channel positioned superior the set of chambers and directly fluidly coupled to the set of chambers, whereby fluid received at the inlet channel concurrently accesses each of the chambers in the set of chambers at the open surface of each chamber; and a fluid delivery module fluidly coupled to the inlet channel operable and configured to distribute a reagent fluid in a direction substantially parallel to the broad surface through the inlet channel, permitting access of the reagent fluid to the set of chambers. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification