Microfluidic device for full blood count

• US 10,191,054 B2
• Filed: 01/26/2010
• Issued: 01/29/2019
• Est. Priority Date: 01/27/2009
• Status: Active Grant

• Current Assignee: Koninklijke Philips Electronics N.V. (Koninklijke Philips N.V.)
• Original Assignee: Koninklijke Philips N.V.
• Inventors: Pettigrew, David M., Gwyer, James D.
• Primary Examiner(s): Brown, Melanie Yu
• Assistant Examiner(s): Moerschell, Richard
• Application Number:
  US13/146,324
• Publication Number:
  US 20110275111A1
• Time in Patent Office: 3,290 Days
• Field of Search: None
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/0627 Sensor or part of a sensor is integrated
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 3/502776 specially adapted for focusing or laminating flows
• CPC Class Codes: G01N 15/12 by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
• CPC Class Codes: G01N 15/1484 microstructural devices
• CPC Class Codes: G01N 2015/011 with lysing, e.g. of erythrocytes
• CPC Class Codes: G01N 2015/016 White blood cells
• CPC Class Codes: G01N 2015/018 Platelets
• CPC Class Codes: G01N 2015/1019 Associating Coulter-counter and optical flow cytometer [OFC]
• CPC Class Codes: G01N 2015/1024 Counting particles by non-optical means
• CPC Class Codes: G01N 33/56972 White blood cells
• CPC Class Codes: G01N 33/721 Haemoglobin
• CPC Class Codes: G01N 33/80 involving blood groups or blood types or red blood cells white blood cells G01N33/56972
• CPC Class Codes: Y10T 137/0402 Cleaning, repairing, or assembling

Abstract

A device for full blood count includes first channel and second channels separated from each other. The device further includes a first inlet configured to provide a whole blood sample to the first and second channels, a second inlet configured to provide a lysis agent for white blood cell count in to the first channel, a third inlet configured to provide a quench solution to the first channel, and a fourth inlet configured to provide a lysis agent for hemoglobin measurement to the second channel.

Claims

1. A microfluidic device for performing a full blood count from a whole blood sample from a finger-prick, the full blood count including measurements of white blood cell differential count, platelet count, red blood cell counts and hemoglobin, the microfluidic device comprising:

a first inlet for providing the whole blood sample;

a second inlet for providing a first lysis agent to count white blood cells, the second inlet being located downstream from the first inlet and being connected to a first junction;

a third inlet for providing a quench solution, the third inlet being located downstream from the second inlet and being connected to a second junction;

a first pair of channels connected to and split from the first inlet and meeting at the first junction,

a second pair of channels connected to and split from the third inlet and meeting at the second junction;

a first snake mixing stage configured to mix the first lysis agent and the whole blood sample, the first snake mixing stage being located between the second and third inlets;

a second snake mixing stage configured to mix the quench solution and the mixture of the first lysis agent and the whole blood sample mixed at the first snake mixing stage, the second snake mixing stage being serially connected to the first snake mixing stage and located downstream from the first snake mixing stage and the third inlet, wherein an input of the first snake mixing stage is connected to the first junction, to the second inlet and to the first pair of channels, and wherein an output of the first snake mixing stage is connected to the second junction, to an input of the second snake mixing stage, and to the second pair of channels for providing the quench solution to the input of the second snake mixing stage from the second pair of channels split from the third inlet and meeting at the second junction; and

a channel for providing a second lysis agent to measure the hemoglobin, the channel including a fourth inlet located downstream from the first inlet for providing the second lysis agent into the channel to measure the hemoglobin.

2. The microfluidic device according to claim 1, further comprising a first sensor that determines the white blood cell differential count at an end of the first channel.

3. The microfluidic device according to claim 2, wherein the first sensor measures impedance.

4. The microfluidic device according to claim 3, further comprising a second sensor that determines properties of red blood cells at an end of the second channel.

5. The microfluidic device according to claim 4, wherein the second sensor is optical.

6. The microfluidic device according to claim 5, further comprising a microfluidic chamber in between the second channel and the second sensor.

7. The microfluidic device according to claim 1, further comprising a microfluidic diluter.

8. The microfluidic device according to claim 1, further comprising a single hydrodynamic pump.

9. The microfluidic device of claim 1, wherein the second inlet is connected to an end of the first snake mixing stage near the first inlet and is for providing the first lysis agent to the first snake mixing stage of first channel to count the white blood cells, and wherein the third inlet is connected to an end of the second snake mixing stage between the first snake mixing stage and the second snake mixing stage.

10. The microfluidic device of claim 1, wherein the volume is between 10 μ

l to 50 μ

l.

11. The microfluidic device of claim 1, wherein a flow rate of the whole blood sample at the first inlet is about 3 μ

l/min, a flow rate of the first lysis agent at the second inlet is about 37 μ

l/min and a flow rate of the quench solution at the third inlet is about 16.3 μ

l/min.

12. The microfluidic device of claim 11, wherein the first lysis agent and the whole blood sample are mixed by diffusion over a length of the first snake mixing stage, the length enables a time of contact between the first lysis agent and the blood sample to be between 5 and 7 seconds.

13. The microfluidic device of claim 1, wherein at least one of the first quench channel and the second quench channel meeting at the junction forms an acute angle with a portion of the first channel downstream of the junction.