Reduction of gastrointestinal tract colonisation by Campylobacter

• US 10,195,172 B2
• Filed: 03/14/2017
• Issued: 02/05/2019
• Est. Priority Date: 02/16/2012
• Status: Active Grant

• Current Assignee: Akeso Biomedical, Inc.
• Original Assignee: Akeso Biomedical, Inc.
• Inventors: Mahdavi, Jafar, Ala'Aldeen, Dlawer
• Primary Examiner(s): Maier, Leigh C
• Application Number:
  US15/458,177
• Publication Number:
  US 20170231945A1
• Time in Patent Office: 693 Days
• CPC Class Codes: A23K 20/111 Aromatic compounds
• CPC Class Codes: A23K 20/142 Amino acids; Derivatives thereof
• CPC Class Codes: A23K 50/75 for poultry
• CPC Class Codes: A61K 31/132 having two or more amino groups, e.g. spermidine, putrescine
• CPC Class Codes: A61K 31/136 having the amino group directly attached to the aromatic ring, e.g. benzeneamine
• CPC Class Codes: A61K 31/164 of a carboxylic acid with an aminoalcohol, e.g. ceramides
• CPC Class Codes: A61K 31/191 having two or more hydroxy groups, e.g. gluconic acid
• CPC Class Codes: A61K 31/198 Alpha-amino acids, e.g. alanine or edetic acid [EDTA] betaine A61K31/205; proline A61K31/401; tryptophan A61K31/405; histidine A61K31/4172; peptides not degraded to individual amino acids A61K38/00
• CPC Class Codes: A61K 31/295 Iron group metal compounds
• CPC Class Codes: A61K 31/335 having oxygen as the only ring hetero atom, e.g. fungichromin
• CPC Class Codes: A61K 31/555 containing heavy metals, e.g. hemin, hematin, melarsoprol
• CPC Class Codes: A61K 31/661 Phosphorus acids or esters thereof not having P—C bonds, e.g. fosfosal, dichlorvos, malathion or mevinphos
• CPC Class Codes: A61K 31/7008 Compounds having an amino group directly attached to a carbon atom of the saccharide radical, e.g. D-galactosamine, ranimustine
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: C12Q 1/18 Testing for antimicrobial activity of a material
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

Campylobacter are the commonest reported bacterial causes of gastroenteritis in the UK and industrialized worlds. This invention relates to a method of preventing or reducing the colonization of the gastrointestinal tract of an animal with Campylobacter. Accordingly, the present invention provides a method for disinfection of an animal comprising administering to said animal at least one compound that binds to MOMP or FlaA of Campylobacter in an effective amount to reduce the number of Campylobacter present in the gastrointestinal tract of said animal. The present invention also provides a method of preventing or reducing transmission of Campylobacter from one animal to another.

Claims

1. An animal feed comprising a complex of tyrosine with Fe III in a concentration from 34 to 340 μ

M effective to inhibit biofilm formation by Campylobacter present in the gastrointestinal tract of an animal or in a meat product.

2. The composition of claim 1, effective to inhibit biofilm formation by Campylobacter present in the gastrointestinal tract of an animal.

3. The composition of claim 1, wherein the animal is poultry.

4. The composition of claim 3, wherein the poultry is selected from the group consisting of chicken, geese, turkeys and ducks.

5. The composition of claim 4, wherein the animal is a chicken.

6. The composition of claim 1, wherein the animal is cattle, sheep, pig or goat.

7. The composition of claim 1, wherein the complex specifically binds to at least one amino acid residue selected from the group consisting of Arg^3S2, Thr²⁶⁸, Lys²⁷⁸, Lys³⁸⁵, Asn²⁵⁸, Leu²⁹⁰, Tyr²⁹⁴, Phe³⁹⁵, Ile³³⁷, Arg³⁸¹, Asp²⁶¹, and Ser³⁹⁷ of MOMP (SEQ ID NO:

1).

8. The composition of claim 6, wherein the complex specifically binds to at least amino acid residue Thr²⁶⁸ of MOMP (SEQ ID NO.1).

9. The composition of claim 1, wherein the complex reduces the campylobacter colony forming units in the gastrointestinal tract of the animal by at least 50%.

10. The composition of claim 1, wherein the Campylobacter is Campylobacter jejuni or Campylobacter coli.

11. A kit comprising the composition of claim 1 and optionally instructions for using the kit.

12. The kit of claim 11, wherein the animal is poultry and is selected from the group consisting of chicken, geese, turkeys and ducks.

13. The kit of claim 11, wherein the animal is cattle, sheep, pig or goat.