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Cartridge-based thermocycler

  • US 10,195,610 B2
  • Filed: 03/09/2015
  • Issued: 02/05/2019
  • Est. Priority Date: 03/10/2014
  • Status: Active Grant
First Claim
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1. A method for performing polymerase chain reaction (PCR), comprising:

  • conveying a sample having a starting volume of at least 50 μ

    L into a first chamber portion of an amplification module, the first chamber portion having a first average temperature;

    producing a flow of the sample from the first chamber portion to a second chamber portion of the amplification module, the second chamber portion having a second average temperature, the second average temperature different than the first average temperature such that the sample is thermally cycled when moved from the first chamber portion to the second chamber portion, the amplification module being constructed from a plurality of layers to define the first chamber portion and the second chamber portion, the plurality of layers including at least a top layer, a bottom layer, a first adhesive layer coupled to the top layer, and a second adhesive layer coupled to the bottom layer, wherein at least one layer selected from the group consisting of the top layer and the bottom layer, comprises poly (4,4′

    -oxydiphenylene-pyromellitimide); and

    completing the PCR of the sample by repeatedly flowing the sample between the first chamber portion and the second chamber portion.

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