Cartridge-based thermocycler
First Claim
Patent Images
1. A method for performing polymerase chain reaction (PCR), comprising:
- conveying a sample having a starting volume of at least 50 μ
L into a first chamber portion of an amplification module, the first chamber portion having a first average temperature;
producing a flow of the sample from the first chamber portion to a second chamber portion of the amplification module, the second chamber portion having a second average temperature, the second average temperature different than the first average temperature such that the sample is thermally cycled when moved from the first chamber portion to the second chamber portion, the amplification module being constructed from a plurality of layers to define the first chamber portion and the second chamber portion, the plurality of layers including at least a top layer, a bottom layer, a first adhesive layer coupled to the top layer, and a second adhesive layer coupled to the bottom layer, wherein at least one layer selected from the group consisting of the top layer and the bottom layer, comprises poly (4,4′
-oxydiphenylene-pyromellitimide); and
completing the PCR of the sample by repeatedly flowing the sample between the first chamber portion and the second chamber portion.
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Abstract
Cartridge-based thermocyclers can include cartridges that are configured to move a fluid between distinct chambers. In some cases, the cartridge-based thermocyclers can be used for thermocycling a sample fluid comprising a deoxyribonucleic acid (DNA) target to perform polymerase chain reaction (PCR). Individual chambers can be heated, cooled, and/or compressed to mix fluid within the chamber or to propel fluid in the chamber into another chamber. The cartridges can have a laminate construction. The cartridges can be configured to enable multiplexed thermocycling and/or detection.
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Citations
22 Claims
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1. A method for performing polymerase chain reaction (PCR), comprising:
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conveying a sample having a starting volume of at least 50 μ
L into a first chamber portion of an amplification module, the first chamber portion having a first average temperature;producing a flow of the sample from the first chamber portion to a second chamber portion of the amplification module, the second chamber portion having a second average temperature, the second average temperature different than the first average temperature such that the sample is thermally cycled when moved from the first chamber portion to the second chamber portion, the amplification module being constructed from a plurality of layers to define the first chamber portion and the second chamber portion, the plurality of layers including at least a top layer, a bottom layer, a first adhesive layer coupled to the top layer, and a second adhesive layer coupled to the bottom layer, wherein at least one layer selected from the group consisting of the top layer and the bottom layer, comprises poly (4,4′
-oxydiphenylene-pyromellitimide); andcompleting the PCR of the sample by repeatedly flowing the sample between the first chamber portion and the second chamber portion. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method for performing polymerase chain reaction (PCR), comprising:
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conveying a sample having a starting volume of at least 50 μ
L into an amplification module having a first portion having and a second portion, the amplification module being constructed from a plurality of layers to define the first portion and the second portion, the plurality of layers including at least a top layer, a bottom layer, a first adhesive layer coupled to the top layer, and a second adhesive layer coupled to the bottom layer, wherein at least one layer selected from the group consisting of the top layer and the bottom layer, comprises poly (4,4′
-oxydiphenylene-pyromellitimide);heating the first portion of the amplification module to a first average temperature; heating the second portion of the amplification module to a second average temperature, the second average temperature different than the first average temperature; producing a flow of the sample from the first portion to the second portion of the amplification module such that the sample is thermally cycled when moved from the first portion to the second portion, and such that the sample transitions from the first average temperature to substantially the second average temperature at a rate of 10 μ
L°
C./second or more; andcompleting the PCR of the sample by repeatedly flowing the sample between the first portion and the second portion at least 20 times. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification