Epitope focusing by variable effective antigen surface concentration
First Claim
1. A method for eliciting an immune response in a human subject, the method comprising:
- delivering at least six antigens to the human subject, wherein each of the at least six antigens comprises;
a target epitope that is common to each of the at least six antigens; and
one or more non-conserved regions that are outside of the target epitope;
wherein the at least six antigens are delivered such that each individual antigen of the at least six antigens is delivered in an amount that is insufficient to be immunogenic to the human subject on its own, while the at least six antigens are delivered in a combined amount that is sufficient to generate an immune response to the target epitope in the human subject.
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Accused Products
Abstract
The present disclosure provides compositions and methods for the generation of an antibody or immunogenic composition, such as a vaccine, through epitope focusing by variable effective antigen surface concentration. Generally, the composition and methods of the disclosure comprise three steps: a “design process” comprising one or more in silico bioinformatics steps to select and generate a library of potential antigens for use in the immunogenic composition; a “formulation process”, comprising in vitro testing of potential antigens, using various biochemical assays, and further combining two or more antigens to generate one or more immunogenic compositions; and an “administering” step, whereby the immunogenic composition is administered to a host animal, immune cell, subject or patient. Further steps may also be included, such as the isolation and production of antibodies raised by host immune response to the immunogenic composition.
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Citations
20 Claims
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1. A method for eliciting an immune response in a human subject, the method comprising:
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delivering at least six antigens to the human subject, wherein each of the at least six antigens comprises; a target epitope that is common to each of the at least six antigens; and one or more non-conserved regions that are outside of the target epitope; wherein the at least six antigens are delivered such that each individual antigen of the at least six antigens is delivered in an amount that is insufficient to be immunogenic to the human subject on its own, while the at least six antigens are delivered in a combined amount that is sufficient to generate an immune response to the target epitope in the human subject. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification