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Multipartite signaling proteins and uses thereof

  • US 10,196,444 B2
  • Filed: 07/23/2014
  • Issued: 02/05/2019
  • Est. Priority Date: 07/29/2013
  • Status: Active Grant
First Claim
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1. An isolated non-natural cell, comprising:

  • (a) a first nucleic acid molecule encoding a first fusion protein comprising a first multimerization domain, a transmembrane domain, and an actuator domain, wherein the first multimerization domain localizes extracellularly when the first fusion protein is expressed; and

    (b) a second nucleic acid molecule encoding a second fusion protein comprising a secretion signal, a binding domain that comprises a single chain antibody, a receptor ectodomain, or a ligand, and a second multimerization domain, wherein the second fusion protein localizes extracellularly when expressed;

    wherein a bridging factor promotes the formation of a polypeptide complex on the non-natural cell surface with the bridging factor associated with and disposed between the multimerization domains of the first and second fusion proteins.

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