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Affinity tag nucleic acid and protein compositions, and processes for using same

  • US 10,196,672 B2
  • Filed: 07/14/2016
  • Issued: 02/05/2019
  • Est. Priority Date: 12/20/2007
  • Status: Active Grant
First Claim
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1. A process for isolating an analyte of interest, said process comprising the steps of:

  • providing(i) a sample containing or suspected of containing said analyte of interest;

    (ii) a first antibody having an affinity for said analyte;

    (iii) a fusion antibody comprising;

    (a) an antibody and at least one affinity peptide attached to the amino terminus or the carboxyl terminus of said antibody, wherein said affinity peptide comprises a metal binding peptide or is one member of a peptide affinity group, wherein said antibody has an affinity for the first antibody;

    or(b) an antibody and an affinity peptide attached to the amino terminus and an affinity peptide attached to carboxyl terminus of said antibody, wherein said affinity peptide comprises a metal binding peptide or is one member of a peptide affinity group, wherein said antibody has an affinity for the first antibody; and

    (iv) a matrix comprising a metal or a second member of said affinity peptide group;

    contacting said sample with said first antibody, thereby forming a first complex between said first antibody and any analyte present in said sample;

    contacting said first complex with said fusion antibody, thereby forming a second complex between said first complex and said fusion antibody;

    contacting said second complex with said matrix to bind said second complex to said matrix;

    removing unbound material from said matrix, andreleasing said analyte of interest from said second complex, thereby isolating said analyte of interest.

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