Methods for analyzing nucleic acid
First Claim
1. A method for reducing and/or detecting bias associated with capture, amplification and sequencing of nucleic acid, the method comprising:
- obtaining a sample comprising a nucleic acid template;
tiling a plurality of molecular inversion probes across a portion of the template, wherein immediately adjacent probes hybridize to opposite strands of the nucleic acid template and probes on the same strand hybridize to the template in an overlapping manner, and wherein each molecular inversion probe comprises a unique differentiator tag sequence;
filling-in a region between targeting arms of a plurality of the molecular inversion probes with nucleotides; and
analyzing the filled-in region of a plurality of the probes to obtain sequence information about the nucleic acid template, wherein the sequence information comprises the differentiator tag sequences;
identifying bias based on the sequence information.
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Abstract
The invention generally relates to methods for analyzing nucleic acids. In certain aspects, methods of the invention involve obtaining a sample including a nucleic acid template. A plurality of molecular inversion probes are tiled across a portion of the template. The probes are designed such that immediately adjacent probes hybridize to opposite strands of the nucleic acid template and probes on the same strand hybridize to the template in an overlapping manner. A region between targeting arms of a plurality of the molecular inversion probes is filled-in with nucleotides, and the filled-in region of a plurality of the probes is analyzed to obtain sequence information about the nucleic acid template.
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Citations
15 Claims
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1. A method for reducing and/or detecting bias associated with capture, amplification and sequencing of nucleic acid, the method comprising:
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obtaining a sample comprising a nucleic acid template; tiling a plurality of molecular inversion probes across a portion of the template, wherein immediately adjacent probes hybridize to opposite strands of the nucleic acid template and probes on the same strand hybridize to the template in an overlapping manner, and wherein each molecular inversion probe comprises a unique differentiator tag sequence; filling-in a region between targeting arms of a plurality of the molecular inversion probes with nucleotides; and analyzing the filled-in region of a plurality of the probes to obtain sequence information about the nucleic acid template, wherein the sequence information comprises the differentiator tag sequences; identifying bias based on the sequence information. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification