Fiber sampler for recovery of bioaerosols and particles

• US 10,208,331 B2
• Filed: 04/27/2018
• Issued: 02/19/2019
• Est. Priority Date: 08/17/2010
• Status: Active Grant

• Current Assignee: Research Triangle Institute
• Original Assignee: Research Triangle Institute
• Inventors: Ensor, David Samuel, Walls, Howard Jerome, Foarde, Karin K.
• Primary Examiner(s): Hixson, Christopher Adam
• Assistant Examiner(s): Berkeley, Emily R.
• Application Number:
  US15/965,040
• Publication Number:
  US 20180245126A1
• Time in Patent Office: 298 Days
• Field of Search: 435 30
• CPC Class Codes: B01D 2239/025 comprising nanofibres apparatus incorporating such gas filtering material see B01D46/546
• CPC Class Codes: B01D 2239/0604 Arrangement of the fibres in the filtering material
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: C12Q 1/24 Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
• CPC Class Codes: G01N 1/2205 with filters
• CPC Class Codes: G01N 1/2208 with impactors
• CPC Class Codes: G01N 1/2214 by sorption
• CPC Class Codes: G01N 2001/2223 aerosol sampling devices
• CPC Class Codes: G01N 33/497 of gaseous biological material, e.g. breath

Abstract

A bioparticle collection device and an aerosol collection system. The bioparticle collection device includes a collection medium including a plurality of fibers formed into a fiber mat and configured to collect bioparticles thereon, and includes a viability enhancing material provider disposed in a vicinity of the plurality of fibers and configured to provide a viability enhancing material to the collected bioparticles to maintain viability of the bioparticles collected by the fiber mat. The aerosol collection system includes an aerosol pumping device configured to entrain particles in an gas stream, an aerosol saturation device configured to saturate the particles in the gas stream with a biocompatible liquid, and an aerosol collection medium downstream from the aerosol saturation device and including a plurality of fibers formed into a fiber mat for collection of the saturated aerosol particles.

Claims

1. An aerosol collection system comprising:

an aerosol pump by which bioparticles in a gas stream are pumped through the system;

a fiber mat disposed in the gas stream for collection of the bioparticles from the gas stream;

a humidifier having a humidifying section in which a relative humidity is controlled, wherein the fiber mat is disposed within the humidifying section thereof; and

a nutrient supply which supplies nutrients directly into the fiber mat,

wherein at least one of the relative humidity of the fiber mat and the nutrient supply into the fiber mat maintains viability of the bioparticles while in the fiber mat both during and after collection of the bioparticles therein.

2. The system of claim 1, wherein the humidifier controls the relative humidity of the fiber mat from 50 to 100%.

3. The system of claim 1, wherein the humidifier controls the relative humidity of the fiber mat from 65 to 85%.

4. The system of claim 1, wherein the nutrient supply comprises an osmotic material disposed in contact with a plurality of fibers of the fiber mat.

5. The system of claim 1, wherein the nutrient supply comprises a supply of at least one of proteins, sugars, and salts.

6. The system of claim 1, wherein the fiber mat comprises polymeric fibers.

7. The system of claim 6, wherein the polymeric fibers comprise materials dissolvable in a bio-compatible solvent.

8. The system of claim 1, wherein the fiber mat comprises polymeric nanofibers.

9. The system of claim 8, wherein the polymeric nanofibers comprise materials dissolvable in a bio-compatible solvent.

10. The system of claim 1, further comprising a support supporting the fiber mat in the gas stream.

11. The system of claim 10, wherein the support comprises:

at least one of a filter, a plastic foam, a metallic foam, a semi-conductive foam, a woven material, a nonwoven material, a plastic screen, a textile, and a high efficiency particulate air (HEPA) filter medium.

12. The system of claim 1, wherein the fiber mat comprises a pH sensitive material.

13. The system of claim 1, wherein the fiber mat comprises a viability enhancing material intermixed with fibers of the fiber mat.

14. The system of claim 13, wherein the viability enhancing material comprises a separate layer from a layer including the fibers.

15. The system of claim 13, wherein the fibers have at least one of hydrophilic or hydrophobic surfaces.

16. The system of claim 13, wherein the fiber mat comprises a hydrogel.

17. The system of claim 13, wherein the fibers comprise at least two different fibers composed respectively of different polymers.

18. The system of claim 13, wherein the fiber mat comprises an antioxidant.

19. The system of claim 13, wherein the fibers are composed from at least one of polysulfone, polyurethane, nylon, polycaprolactone, and polystyrene.

20. The system of claim 13, wherein the fibers comprise a material having a higher bioparticle viability than polytetrafluoroethylene.