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Methods and systems for processing polynucleotides

  • US 10,208,343 B2
  • Filed: 12/05/2017
  • Issued: 02/19/2019
  • Est. Priority Date: 06/26/2014
  • Status: Active Grant
First Claim
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1. A library of partitions for barcoding nucleic acid molecules, comprising:

  • a plurality of partitions comprising a plurality of gel beads that are degradable upon application of a stimulus, wherein said plurality of gel beads comprises a plurality of barcode sequences that are different across said plurality of gel beads,wherein a given partition of said plurality of partitions comprises a given gel bead of said plurality of gel beads and an agent configured to degrade said given gel bead,wherein said given gel bead of said plurality of gel beads comprises a plurality of nucleic acid molecules, andwherein each of said plurality of nucleic acid molecules comprises a barcode sequence from said plurality of barcode sequences and a unique molecular sequence, wherein (i) said barcode sequence is constant across said plurality of nucleic acid molecules, and (ii) said unique molecular sequence varies across said plurality of nucleic acid molecules.

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