Methods and systems for assessing infertility and related pathologies
First Claim
1. A method for assessing endometriosis, the method comprising:
- sequencing nucleic acid obtained from a tissue sample to determine levels of transcripts of a plurality of genes present in a tissue sample obtained from a subject suspected of having endometriosis, the plurality of genes comprising CCL 3L 1, CCL 3, FAM 180A, THBS 2, PDGFRL, FN 1, CLE 11A, CCNA 2, KIF 20A, BUB 1B, HSD 17B 6, HSD 11B 1, C 7, C 3, CXCL 2, CXCL 12, CXCL 13, PDGFC, CXCL 14, ACTA 2, TAGLN, ROBO 3, MT 1M, and SORBS 1;
analyzing said levels against a reference regulation pattern specific to a time-point in a uterine cycle using a computer system having a processor, thereby to generate a patient-specific signature;
preparing a report characterizing an endometriosis status of the subject based upon said patient-specific signature; and
treating the subject with an effective amount of drugs specific to the patient-specific signature;
wherein the reference regulation pattern is generated using gene expression data obtained from the plurality of genes across each phase of the uterine cycle from a reference population that includes both normal patients and endometriosis patients.
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Abstract
Methods for assessing infertility and related pathologies and informing treatment type and timing thereof are provided. According to certain embodiments, methods of the invention include determining levels of one or more transcripts present in a sample obtained from a subject suspected of having endometriosis, identifying transcript levels that correspond to a regulation pattern specific to a time-point in a uterine cycle, and characterizing endometriosis of the subject based upon the identified transcript levels. The invention includes methods for assessing age-associated increase in aneuploidy rates based on FSH levels and IVF success rates based on obesity in PCOS patients.
59 Citations
17 Claims
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1. A method for assessing endometriosis, the method comprising:
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sequencing nucleic acid obtained from a tissue sample to determine levels of transcripts of a plurality of genes present in a tissue sample obtained from a subject suspected of having endometriosis, the plurality of genes comprising CCL 3L 1, CCL 3, FAM 180A, THBS 2, PDGFRL, FN 1, CLE 11A, CCNA 2, KIF 20A, BUB 1B, HSD 17B 6, HSD 11B 1, C 7, C 3, CXCL 2, CXCL 12, CXCL 13, PDGFC, CXCL 14, ACTA 2, TAGLN, ROBO 3, MT 1M, and SORBS 1; analyzing said levels against a reference regulation pattern specific to a time-point in a uterine cycle using a computer system having a processor, thereby to generate a patient-specific signature; preparing a report characterizing an endometriosis status of the subject based upon said patient-specific signature; and treating the subject with an effective amount of drugs specific to the patient-specific signature; wherein the reference regulation pattern is generated using gene expression data obtained from the plurality of genes across each phase of the uterine cycle from a reference population that includes both normal patients and endometriosis patients. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method for assessing endometriosis in a subject, the method comprising:
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sequencing nucleic acid obtained from a blood sample to determine levels of transcripts of a plurality of genes expressed by the subject across different time-points of the subject'"'"'s uterine cycle, the plurality of genes comprising CCL 3L 1, CCL 3, FAM 180A, THBS 2, PDGFRL, FN 1, CLE 11A, CCNA 2, KIF 20A, BUB 1B, HSD 17B 6, HSD 11B 1, C 7, C 3, CXCL 2, CXCL 12, CXCL 13, PDGFC, CXCL 14, ACTA 2, TAGLN, ROBO 3, MT 1M, and SORBS 1; analyzing the determined transcript levels against reference transcript signatures corresponding to the different time-points of the uterine cycle using a computer system having a processor, wherein the reference transcript signatures are generated using gene expression data obtained from the plurality of genes across each phase of the uterine cycle from a reference population that includes both normal patients and endometriosis patients; generating, using the computer system, a patient-specific genetic signature for endometriosis at each time-point based on differential transcripts of the genes determined from the analyzing step; classifying endometriosis based on the patient-specific genetic signature; and treating the subject with a treatment specific to the patient-specific signature, wherein the treatment comprises administering an effective amount of vitamins or drugs. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17)
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Specification