System and method for processing biological specimens

• US 10,209,166 B2
• Filed: 11/18/2015
• Issued: 02/19/2019
• Est. Priority Date: 11/18/2014
• Status: Active Grant

• Current Assignee: Alfonso Heras, Jack Novak, Vargas, Jose
• Original Assignee: Alfonso Heras, Jack Novak, Vargas, Jose
• Inventors: Heras, Alfonso, Vargas, Jose, Novak, Jack
• Primary Examiner(s): Nagpaul, Jyoti
• Application Number:
  US14/945,264
• Publication Number:
  US 20160139010A1
• Time in Patent Office: 1,189 Days
• CPC Class Codes: B01L 2300/0822 Slides
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 9/52 Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: G01N 2035/00138 Slides
• CPC Class Codes: G01N 2035/00237 Handling microquantities of analyte, e.g. microvalves, capillary networks
• CPC Class Codes: G01N 2035/00356 Holding samples at elevated temperature (incubation)
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence
• CPC Class Codes: G02B 21/34 Microscope slides, e.g. mounting specimens on microscope slides

Abstract

A system for processing biological specimens mounted on microscope slides by adding and removing processing fluids from microscope slides by means of capillary action using a slide holder capable of holding multiple microscope slides, and a spacer positioned in between two slides of a slide pair to create a capillary gap. A capillary gap adjuster can be used to pinch and release one end of the slide pair to create a pulsatile action to mix the reagent within the capillary gap. The system may further include a reagent holder, an absorbent pad, a series of reagent baths, and an incubator for holding one or more slide holders.

Claims

1. A system for processing biological specimens, comprising:

a. a plurality of slides;

b. a plurality of spacers, one spacer placed in between two slides to create a slide pair, the slide pair having a pair of inner surfaces adjacent to each other and separated by a capillary gap, and a pair of outer surfaces on opposing sides;

c. a slide holder, comprising;

i. a plurality of posts serially arranged on a cover, wherein a groove is defined in between adjacent posts for receiving one slide pair,

ii. a plurality of retaining springs, wherein for each slide pair, a first retaining spring biases against a first post and a first outer surface of the slide pair,

iii. a capillary gap adjuster, comprising a actuator, a nut operatively connected to the actuator such that movement of the actuator in a first direction rotates the nut in a first rotational direction, and movement of the actuator in a second direction rotates the nut in a second rotational direction, a piston operatively connected to the nut, wherein rotation of the nut in the first rotational direction causes linear movement of the piston in a first linear direction, and rotation of the nut in the second rotational direction causes linear movement of the piston in a second linear direction opposite the first linear direction, and a slide rack operatively connected to the piston, wherein movement of the piston in the first linear direction causes movement of the slide rack in a third linear direction, and movement of the piston in the second linear direction causes movement of the slide rack in a fourth linear direction, wherein the slide rack is operatively connected to the slide pair such that movement of the slide rack in the third linear direction causes a first end of the slide pair to pinch together, and movement of the slide rack in the fourth linear direction allows the first end of the slide pair to move apart, wherein when the first end of the slide pair is pinched together the capillary gap grows and wherein when the first end of the slide pair moves apart, the capillary gap shrinks;

d. a reagent tray comprising a plurality of troughs, wherein the troughs are spaced apart to correspond with each of the slide pairs on the slide holder so that each slide pair can be dipped into one trough simultaneously;

e. a reagent bath configured to receive all slide pairs on the slide holder, the reagent bath defining an opening substantially similar in size to the cover such that the cover can cap the opening when the slide pairs are inside the reagent bath;

f. an incubator for heating the reagent bath; and

g. an absorbent pad for removing a reagent from the capillary gap when the slide pair contacts the absorbent pad.

2. A system for exposing a biological specimen to a reagent, comprising:

a. a slide holder comprising;

i. a cover;

ii. a plurality of posts serially arranged along the cover, wherein a groove is defined in between adjacent posts for receiving one slide pair; and

iii. a plurality of retaining springs for securing slide pairs against their respective posts;

b . a plurality of spacers for separating slides within the slide pairs to create a capillary gap between the slides of each slide pair, wherein for each spacer, the spacer is positioned in between a first end of the respective slide pair and the second end of the respective slide pair to function as a fulcrum to allow the capillary gap at the second end of the slide pair to grow and shrink.

3. The system of claim 2, wherein the slide holder further comprises a capillary gap adjuster for adjusting the capillary.

4. The system of claim 3, wherein the capillary gap adjuster comprises:

a. an actuator;

b. a nut operatively connected to the actuator such that movement of the actuator in a first direction rotates the nut in a first rotational direction, and movement of the actuator in a second direction rotates the nut in a second rotational direction;

c. a piston operatively connected to the nut, wherein rotation of the nut in the first rotational direction causes linear movement of the piston in a first linear direction, and rotation of the nut in the second rotational direction causes linear movement of the piston in a second linear direction opposite the first linear direction; and

d. a slide rack operatively connected to the piston, wherein movement of the piston in the first linear direction causes movement of the slide rack in a third linear direction, and movement of the piston in the second linear direction causes movement of the slide rack in a fourth linear direction, wherein the slide rack is operatively connected to each slide pair such that movement of the slide rack in the third linear direction causes the first end of each slide pair to pinch together, and movement of the slide rack in the fourth linear direction allows the first end of the slide pair to move apart, wherein when the first end of the slide pair is pinched together the capillary gap at the second end grows and wherein when the first end of the slide pair moves apart, the capillary gap at the second end shrinks.

5. The system of claim 4, further comprising a reagent tray comprising a plurality of troughs, wherein the troughs are arranged to correspond to the second ends of certain slide pairs on the slide holder.

6. The system of claim 5, wherein the plurality of troughs are arranged in sets and sized to fit a single slide pair, arranged so that each trough will correspond to a single slide pair.

7. The system of claim 4, further comprising a reagent bath, wherein the reagent bath comprises a container sized to accommodate the second ends of all the slide pairs.

8. The system of claim 7, wherein the reagent bath defines an opening dimensioned similar to the cover to permit the cover to cap the opening.

9. The system of claim 4, further comprising an incubator comprising compartments operatively connected to a heating element to heat the compartment, wherein the compartment is sized to accommodate the second ends of a plurality of slide pairs in the slide holder.

10. The system of claim 9, wherein the compartment is dimensioned to receive a reagent bath that is sized to accommodate the second ends of a plurality of slide pairs in the slide holder.

11. The system of claim 4, further comprising an absorbent pad.

12. The system of claim 11, wherein the absorbent pad comprises a plurality of wells arranged in sets, wherein each set comprises wells spaced apart to correspond with each slide pair.

13. The system of claim 2, further comprising a reagent tray comprising a plurality of troughs, wherein the troughs are arranged to correspond to the second ends of certain slide pairs on the slide holder.

14. The system of claim 13, wherein the plurality of troughs are arranged in sets and sized to fit a single slide pair, arranged so that each trough will correspond to a single slide pair.

15. The system of claim 2, further comprising a reagent bath, wherein the reagent bath comprises a container sized to accommodate the second ends of all the slide pairs.

16. The system of claim 15, wherein the reagent bath defines an opening dimensioned similar to the cover to permit the cover to cap the opening.

17. The system of claim 2, further comprising an incubator comprising compartments operatively connected to a heating element to heat the compartment, wherein the compartment is sized to accommodate the second ends of a plurality of slide pairs in the slide holder.

18. The system of claim 17, wherein the compartment is dimensioned to receive a reagent bath that is sized to accommodate the second ends of a plurality of slide pairs in the slide holder.

19. The system of claim 2, further comprising an absorbent pad.

20. The system of claim 19, wherein the absorbent pad comprises a plurality of wells arranged in sets, wherein each set comprises wells spaced apart to correspond with each slide pair.