Biosensor

• US 10,209,214 B2
• Filed: 11/22/2013
• Issued: 02/19/2019
• Est. Priority Date: 03/28/2013
• Status: Active Grant

• Current Assignee: Leadway (Hk) Limited (Akatsuki Corp.)
• Original Assignee: Leadway (Hk) Limited (Akatsuki Corp.)
• Inventors: Zhang, Ziyi, Ge, Huanxi
• Primary Examiner(s): Noguerola, Alexander S
• Application Number:
  US14/775,688
• Publication Number:
  US 20160195490A1
• Time in Patent Office: 1,915 Days
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

A biosensor comprising an insulative substrate (1) and an electrode system arranged on the insulative substrate (1). The electrode system comprises thereon a reagent capable of reacting with a test sample. The electrode system comprises functional electrodes (2, 3, and 4) used for testing and assisted diffusion electrodes (12) used for controlling the diffusion of the reagent. The functional electrodes (2, 3, and 4) are arranged between the assisted diffusion electrodes (12). Because the assisted diffusion electrode (12) are capable of overcoming the effect of surface tension of the insulative base board, the reagent is allowed to diffuse smoothly and uniformly on the electrode system and to arrive at a predetermined position.

Claims

1. A biosensor, comprising:

an insulative substrate and an electrode system on the insulative substrate,

wherein the electrode system comprises a reaction reagent that can react with samples to be detected thereon, functional electrodes for detection, and assisted diffusion electrodes for controlling the diffusion of the reaction reagent, wherein the functional electrodes are located between the assisted diffusion electrodes, and

an insulating layer that covers part of the electrode system, the insulating layer having a hole forming a closed region, wherein the reaction reagent is located in the closed region on the electrode system, the closed region is surrounded by the insulating layer and comprises a closed boundary without any sharp angle, and the reaction reagent covering the closed region without extending beyond the closed boundary.

2. The biosensor of claim 1, wherein there are at least two assisted diffusion electrodes.

3. The biosensor of claim 2, wherein the at least two assisted diffusion electrodes are respectively located at the outsides of the functional electrodes.

4. The biosensor of claim 1, wherein the functional electrodes comprise a working electrode, a counter electrode and a reference electrode, and the functional electrodes are arranged in parallel with the assisted diffusion electrodes, and are generally parallel to each other.

5. The biosensor of claim 4, wherein there are two assisted diffusion electrodes, and the functional electrodes are located between the two assisted diffusion electrodes.

6. The biosensor of claim 1, wherein the shape of the closed boundary is selected from the group consisting of a circle, an oval, two relative ovals, or a rectangle with four circular vertex angles.

7. The biosensor of claim 6, wherein the thickness of the insulating layer is from 3-30 micrometers.