Coating process for microfluidic sample arrays

• US 10,213,761 B2
• Filed: 06/09/2014
• Issued: 02/26/2019
• Est. Priority Date: 08/04/2004
• Status: Active Grant

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Brenan, Colin J. H., Cho, Jamie, Garcia, Javier, Hess, Robert, Kanigan, Tanya S., Katz, Arrin, Kim, Namyong, Linton, John, Srivastava, Shailesh, Yoder, Karl
• Primary Examiner(s): Boyer, Randy
• Application Number:
  US14/300,100
• Publication Number:
  US 20150011436A1
• Time in Patent Office: 1,723 Days
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/16 Surface properties and coatings
• CPC Class Codes: B01L 3/0244 using pins
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/50857 using arrays or bundles of open capillaries for holding samples

Abstract

A differentially coated device for conducting a plurality of nano-volume specified reactions, the device comprising a platen having at least one exterior surface modified to a specified physicochemical property, a plurality of nano-volume channels, each nano-volume channel having at least one interior surface in communication with the at least one exterior surface that is selectively coated with an optionally dissolvable coating agent physisorbed to at least one interior surface, wherein the optionally dissolvable coating agent comprises a coating agent and a first component for the plurality of specified reactions. Methods for preparing and using such devices are also provided, as well as a method of registering a location of a dispenser array in relation to a microfluidic array. A first one of the dispenser array and the microfluidic array is movable in relation to the frame, and the other of the first one of the dispenser array and the microfluidic array is fixed relative to the frame. Quantities related to a vector displacement from the alignment position to a fixed position on the one of the dispenser array and the microfluidic array is determined. The quantities thus determined are used to guide positioning of the dispenser array relative to the microfluidic array.

Claims

1. A method for differentially coating a substrate, the method comprising:

supplying a substrate comprising an external surface and a plurality of through-hole channels each comprising an interior surface in communication with the exterior surface;

applying a first coating agent simultaneously to both the exterior surface and to at least one of the interior surfaces;

selectively oxidizing the at least one interior surface with an oxidizing agent;

after selectively oxidizing, selectively modifying the first coating agent applied to the at least one interior surface with a first modifying agent such that, after modifying, the at least one interior surface has a first specified physicochemical property that differs with respect to a specified physicochemical property of the external surface; and

applying a second modifying agent to the at least one interior surface;

wherein at least one of (1) the first coating agent comprises a vinyl terminated compound or (2) the first and second modifying agents each comprise a polyethylene glycol.

4. The method of claim 1, wherein the first coating agent comprises a vinyl terminated compound and the modifying agent comprises a polyethylene glycol.

5. The method of claim 1, further comprising, after applying the second modifying agent, applying a film by vapor deposition onto the external surface, wherein the second modifying agent protects the plurality of through-hole channels.

6. The method of claim 5, further comprising removing the second modifying agent, wherein the at least one interior surfaces comprises a hydrophilic surface and the exterior surface comprises a hydrophobic surface.

7. The method of claim 6, wherein the hydrophobic surface comprises a perfluorosilane film.

2. A method for differentially coating a substrate having an exterior surface and a plurality of through-hole channels each comprising an interior surface in communication with the exterior surface, the method comprising:

creating a coated surface by applying a first coating agent simultaneously to both the exterior surface and to at least one of the interior surfaces;

selectively oxidizing the at least one interior surface with an oxidizing agent; and

after selectively oxidizing, selectively modifying the first coating agent with a first modifying agent such that the coated surface comprises a modified surface and a non-modified surface, the modified surface comprising the at least one interior surface and having a first specified physicochemical property, the non-modified surface comprising a second specified physicochemical property that differs with respect to the first specified physicochemical property;

wherein at least one of (1) the first coating agent comprises a vinyl terminated compound or (2) the first and second modifying agents each comprise a polyethylene glycol.

8. The method of claim 2, wherein the first coating agent comprises a vinyl terminated compound and the modifying agent comprises a polyethylene glycol.

9. The method of claim 2, further comprising applying a second modifying agent to the at least one interior surface.

10. The method of claim 9, further comprising, after applying the second modifying agent, applying a film by vapor deposition onto the external surface, wherein the second modifying agent protects the plurality of through-hole channels.

11. The method of claim 9, further comprising removing the second modifying agent, wherein the at least one interior surfaces comprises a hydrophilic surface and the exterior surface comprises a hydrophobic surface.

12. The method of claim 11, wherein the exterior surface comprises a perfluorosilane film.

3. A method for differentially coating a substrate having an exterior surface and a plurality of through-hole channels each comprising an interior surface in communication with the exterior surface, the method comprising:

creating a coated surface by applying a first coating agent simultaneously to both the exterior surface and to at least one of the interior surfaces;

selectively oxidizing the at least one interior surface with an oxidizing agent; and

after selectively oxidizing, selectively modifying the first coating agent with a first modifying agent such that the coated surface comprises a modified surface and a non-modified surface, the modified surface comprising the at least one interior surface, the unmodified surface comprising exterior surface;

wherein at least one of (1) the first coating agent comprises a vinyl terminated compound or (2) the first and second modifying agents each comprise a polyethylene glycol.

13. The method of claim 3, wherein the first coating agent comprises a vinyl terminated compound and the modifying agent comprises a polyethylene glycol.

14. The method of claim 3, further comprising applying a second modifying agent to the at least one interior surface.

15. The method of claim 14, further comprising, after applying the second modifying agent, applying a film by vapor deposition onto the external surface, wherein the second modifying agent protects the plurality of through-hole channels.

16. The method of claim 14, further comprising removing the second modifying agent, wherein the at least one interior surfaces comprises a hydrophilic surface and the exterior surface comprises a hydrophobic surface.

17. The method of claim 16, wherein the exterior surface comprises a perfluorosilane film.