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Nucleic acid extracting device

  • US 10,213,783 B2
  • Filed: 02/08/2016
  • Issued: 02/26/2019
  • Est. Priority Date: 07/17/2015
  • Status: Active Grant
First Claim
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1. A nucleic acid extracting device, comprising:

  • a lower module, having a sample reservoir, an elution reservoir, and a nucleic acid capturing chamber, wherein a sample is received in the sample reservoir, and an elution is received in the elution reservoir;

    a material for capturing nucleic acid, disposed in the nucleic acid capturing chamber; and

    an upper module, having a sample channel that communicates with the sample reservoir, and an elution channel that communicates with the elution reservoir, wherein the nucleic acid capturing chamber communicates with the sample channel and the elution channel;

    a flexible buffer member disposed between the upper module and the lower module to form an annular rib;

    wherein the sample enters the nucleic acid capturing chamber via the sample channel, a nucleic acid in the sample is absorbed by the material, and the elution enters the nucleic acid capturing chamber via the elution channel to wash the nucleic acid out of the nucleic acid capturing chamber;

    wherein the upper module further has a bottom surface facing toward the lower module, and an annular guide portion protruding from the bottom surface and facing toward the nucleic acid capturing chamber, wherein the elution channel communicates the elution reservoir with the guide portion, the annular rib surrounds the guide portion with a gap formed therebetween, and the height of the guide portion relative to the bottom surface exceeds the height of the rib relative to the bottom surface;

    wherein the sample channel communicates the sample reservoir with the guide portion, and an inner surface of the guide portion forms a plurality of recesses corresponding to the sample channel and the elution channel respectively, wherein each of the recesses forms a bottom and two sidewalls on opposite sides of the bottom to fluidly isolate the recesses from each other, and the width of the bottom exceeds the widths of the sidewalls.

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