Postpartum cells derived from placental tissue, and methods of making and using the same
First Claim
1. An extracellular matrix composition comprising isolated human placenta-derived cells and extracellular matrix produced by a population of the isolated human placenta-derived cells, wherein the isolated human placenta-derived cells are derived from human postpartum placenta tissue substantially free of blood, wherein the placenta-derived cell self-renews and expands in culture, is multipotent, grows in about 5% to about 20% oxygen, and further comprises the following characteristics:
- a) produces CD10, CD13, CD44, CD73, CD90, PDGFr-alpha, PD-L2, HLA-A,B,C, and granulocyte chemotactic protein-2 (GCP-2);
b) does not produce CD31, CD34, CD45, CD80, CD86, CD117, CD141, CD178, B7-H2, HLA-G or HLA-DR-DP, DQ, as detected by flow cytometry; and
c) expresses, relative to a human fibroblast, mesenchymal stem cell, or iliac crest bone marrow cell, increased levels of oxidized low density lipoprotein receptor 1 and renin.
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Abstract
Cells derived from postpartum placenta and methods for their isolation are provided by the invention. The invention further provides cultures and compositions of the placenta-derived cells. The placenta-derived cells of the invention have a plethora of uses, including but not limited to research, diagnostic, and therapeutic applications.
255 Citations
14 Claims
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1. An extracellular matrix composition comprising isolated human placenta-derived cells and extracellular matrix produced by a population of the isolated human placenta-derived cells, wherein the isolated human placenta-derived cells are derived from human postpartum placenta tissue substantially free of blood, wherein the placenta-derived cell self-renews and expands in culture, is multipotent, grows in about 5% to about 20% oxygen, and further comprises the following characteristics:
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a) produces CD10, CD13, CD44, CD73, CD90, PDGFr-alpha, PD-L2, HLA-A,B,C, and granulocyte chemotactic protein-2 (GCP-2); b) does not produce CD31, CD34, CD45, CD80, CD86, CD117, CD141, CD178, B7-H2, HLA-G or HLA-DR-DP, DQ, as detected by flow cytometry; and c) expresses, relative to a human fibroblast, mesenchymal stem cell, or iliac crest bone marrow cell, increased levels of oxidized low density lipoprotein receptor 1 and renin. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification