Systems, methods, and apparatuses to image a sample for biological or chemical analysis

• US 10,220,386 B2
• Filed: 11/22/2014
• Issued: 03/05/2019
• Est. Priority Date: 01/10/2011
• Status: Active Grant

• Current Assignee: Illumina Incorporated
• Original Assignee: Illumina Incorporated
• Inventors: Williamson, Erik, Crane, Bryan, Leung, Patrick, Verkade, Drew, Reed, Mark T.
• Primary Examiner(s): Hurst, Jonathan M
• Application Number:
  US14/550,956
• Publication Number:
  US 20150151297A1
• Time in Patent Office: 1,564 Days
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/04 Exchange or ejection of cartridges, containers or reservoirs
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/022 Transponder chips
• CPC Class Codes: B01L 2300/041 Connecting closures to device or container
• CPC Class Codes: B01L 2300/043 Hinged closures
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: G01N 2021/058 Flat flow cell
• CPC Class Codes: G01N 2035/00435 Refrigerated reagent storage
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
• CPC Class Codes: G01N 35/1002 Reagent dispensers
• CPC Class Codes: G01N 35/1011 Control of the position or alignment of the transfer device
• CPC Class Codes: G01N 35/1065 Multiple transfer devices
• CPC Class Codes: G01N 35/1079 : with means for piercing stoppers or septums
• CPC Class Codes: Y10T 436/25 : including sample preparation

Abstract

A fluidic device holder configured to orient a fluidic device. The device holder includes a support structure configured to receive a fluidic device. The support structure includes a base surface that faces in a direction along the Z-axis and is configured to have the fluidic device positioned thereon. The device holder also includes a plurality of reference surfaces facing in respective directions along an XY-plane. The device holder also includes an alignment assembly having an actuator and a movable locator arm that is operatively coupled to the actuator. The locator arm has an engagement end. The actuator moves the locator arm between retracted and biased positions to move the engagement end away from and toward the reference surfaces. The locator arm is configured to hold the fluidic device against the reference surfaces when the locator arm is in the biased position.

Claims

1. A fluidic device for analyzing samples, the fluidic device comprising:

a flow cell including inlet and outlet ports and a flow channel extending therebetween, the flow cell configured to hold a sample-of-interest;

a housing having a reception space that is configured to receive the flow cell, the reception space being sized and shaped to permit the flow cell to float relative to the housing;

a gasket coupled to the housing, the gasket having inlet and outlet passages and comprising a compressible material, the gasket being positioned relative to the reception space so that the inlet and outlet ports of the flow cell are approximately aligned with the inlet and outlet passages of the gasket, respectively, and

a cover member rotatably coupled to the housing so as to be rotatable about an axis of rotation extending through the housing between a mounted position and a disengaged position;

wherein a top surface of the cover member is substantially flush with a top surface of the housing when the cover member is set in the mounted position.

2. The fluidic device of claim 1, wherein the cover member includes the gasket and a cover material, wherein the cover material and the gasket are co-molded, the compressible material of the gasket being more compressible than the cover material.

3. The fluidic device of claim 1, wherein the cover member includes the gasket and a cover material, the inlet and outlet passages of the gasket being approximately aligned with the inlet and outlet ports when the cover member is in the mounted position.

4. The fluidic device of claim 1, wherein the cover member includes the gasket and a cover material, wherein the housing further comprises fin projections and wherein the cover member extends between the fin projections.

5. The fluidic device of claim 1, wherein the flow cell has a plurality of edges that extend along a cell plane and define a perimeter of the flow cell, the gasket configured to press against one of the edges when the inlet and outlet passages are approximately aligned, the gasket limiting movement of the flow cell within the reception space along the cell plane.

6. The fluidic device of claim 5, wherein the flow cell has first and second cell sides that face in opposite directions, the first and second cell sides extending along the cell plane, wherein the gasket also presses against one of the first and second cell sides thereby limiting movement in a direction that is perpendicular to the cell plane.

7. The fluidic device of claim 6, wherein the gasket is movable with respect to the housing and flow cell, the inlet and outlet passages being approximately aligned with the inlet and outlet ports when the gasket is mounted over the flow cell.

8. The fluidic device of claim 1, wherein the flow channel comprises an imaging portion and a non-imaging portion, wherein imaging and nonimaging portions are fluidicly joined by a curved portion, the imaging and non-imaging portions extending parallel to each other,

wherein the inlet port is in fluid communication with the imaging portion of the flow channel, and wherein the outlet port is in fluid communication with the non-imaging portion of the flow channel.

9. The fluidic device of claim 8, wherein the inlet port and the outlet port are located proximate to each other at one end of the flow cell.

10. The fluidic device of claim 9, wherein the spacing between the inlet port and the outlet port is 3 mm or less.

11. The fluidic device of claim 8, wherein the width of the imaging portion of the flow channel is larger than the width of the non-imaging portion of the flow channel.

12. The fluidic device of claim 1, wherein the length of the substrate is 30 mm or less, the width of the substrate is 15 mm or less and the height of the substrate is 1.5 mm or less.

13. The fluidic device of claim 8, wherein the curved portion of the flow channel comprises a non-continuous contour that fluidicly joins the imaging portion of the flow channel with the non-imaging portion of the flow channel.

14. The fluidic device of claim 13, wherein the curved portion of the flow channel comprises a tapering portion and an intermediate portion,

wherein the tapering portion connects the imaging portion with the intermediate portion, and

wherein the width of the tapering portion reduces in size from the imaging portion of the flow channel to the intermediate portion.

15. The fluidic device of claim 1, further comprising an identification transmitter positioned in a cavity of the housing.

16. The fluidic device of claim 15, wherein the identification transmitter comprises and RFID tag.

17. The fluidic device of claim 1, wherein the housing comprises a loading end and a receiving end, the reception space extends from the loading end and terminates along the housing, and the axis of rotation is proximate the receiving end such that the cover member extends between the reception space and the receiving end when set in the mounted position.