Synthetic surfaces for culturing stem cell derived oligodendrocyte progenitor cells

• US 10,221,390 B2
• Filed: 03/07/2017
• Issued: 03/05/2019
• Est. Priority Date: 01/30/2008
• Status: Active Grant

• Current Assignee: Asterias Biotherapeutics Incorporated (Lineage Cell Therapeutics, Inc.)
• Original Assignee: Asterias Biotherapeutics Incorporated (Lineage Cell Therapeutics, Inc.)
• Inventors: Shogbon, Christopher Bankole, Zhou, Yue, Brandenberger, Ralph
• Primary Examiner(s): Ton, Thaian N
• Application Number:
  US15/452,710
• Publication Number:
  US 20180023053A1
• Time in Patent Office: 728 Days
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12N 2500/90 Serum-free medium, which may still contain naturally-sourced components
• CPC Class Codes: C12N 2506/02 from embryonic cells
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0622 Glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells
• CPC Class Codes: C12N 5/0623 Stem cells

Abstract

Synthetic surfaces suitable for culturing stem cell derived oligodendrocyte progenitor cells contain acrylate polymers formed from one or more acrylate monomers. The acrylate surfaces, in many cases, are suitable for culturing stem cell derived oligodendrocyte progenitor cells in chemically defined media.

Claims

1. A method for culturing human pluripotent stem cell-derived oligodendrocyte progenitor cells, comprising depositing a suspension comprising the human pluripotent stem cell-derived oligodendrocyte progenitor cells on a polymer material, and culturing the deposited human pluripotent stem cell-derived oligodendrocyte progenitor cells in a cell culture medium, wherein the polymer material comprises a diacrylate or a dimethacrylate.

2. The method of claim 1, wherein the dimethacrylate is glycerol dimethacrylate.

3. The method of claim 1, wherein the diacrylate is 1,6-hexanediol diacrylate.

4. The method of claim 1, wherein the diacrylate is tetra(ethylene glycol) diacrylate.

5. The method of claim 1, wherein the polymer material comprises about 70% glycerol dimethacrylate.

6. The method of claim 1, wherein the human pluripotent stem cells are human sells induced pluripotent stem (iPS) cells.

7. The method of claim 1, wherein the human pluripotent stem cells are human embryonic stem (hES) cells.

8. The method of claim 1, wherein the cell culture medium is a chemically defined medium.