Recovery and processing of human embryos formed in vivo

• US 10,226,280 B2
• Filed: 07/30/2015
• Issued: 03/12/2019
• Est. Priority Date: 12/22/2011
• Status: Active Grant

• Current Assignee: Butterfly Biosciences Incorporated
• Original Assignee: Previvo Genetics, LLC
• Inventors: Carson, Sandra Ann, Buster, John E., Cesario, Moses, Woodard, Steven Paul
• Primary Examiner(s): Dorna, Carrie R
• Application Number:
  US14/814,252
• Publication Number:
  US 20150335701A1
• Time in Patent Office: 1,321 Days
• CPC Class Codes: A61B 17/42 Gynaecological or obstetrical instruments or methods ligaturing clamps or clips for the umbilical cord A61B17/122; curettage A61B17/320708; dilators A61M29/00
• CPC Class Codes: A61B 17/425 for reproduction or fertilisation specially adapted for use with animals A61D19/00
• CPC Class Codes: A61B 17/435 for embryo or ova transplantation
• CPC Class Codes: A61B 2017/4216 Operations on uterus, e.g. endometrium
• CPC Class Codes: A61B 2050/185 Drawers
• CPC Class Codes: A61B 2050/3008 having multiple compartments
• CPC Class Codes: A61B 2050/3011 having carrying handles
• CPC Class Codes: A61B 2050/311 Cases
• CPC Class Codes: A61B 2217/005 with suction drainage system
• CPC Class Codes: A61B 2217/007 with irrigation system
• CPC Class Codes: A61B 50/13 Trolleys, e.g. carts
• CPC Class Codes: A61B 50/20 Holders specially adapted for surgical or diagnostic appliances or instruments drapes with means to hold surgical instruments A61B46/23; for catheters A61M25/02
• CPC Class Codes: A61B 50/31 Carrying cases or bags, e.g. doctors' bags
• CPC Class Codes: A61K 31/215 of carboxylic acids
• CPC Class Codes: A61K 31/57 substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane or progesterone
• CPC Class Codes: A61K 31/573 substituted in position 21, e.g. cortisone, dexamethasone, prednisone or aldosterone
• CPC Class Codes: A61K 31/575 substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol
• CPC Class Codes: A61K 31/58 containing heterocyclic rings, e.g. danazol, stanozolol, pancuronium or digitogenin digitoxin A61K31/7048
• CPC Class Codes: A61K 38/08 Peptides having 5 to 11 amino acids A61K38/043 - A61K38/046 take precedence
• CPC Class Codes: A61K 38/09 Luteinising hormone-releasing hormone [LHRH] , i.e. Gonadotropin-releasing hormone [GnRH]; Related peptides
• CPC Class Codes: A61K 38/24 : Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
• CPC Class Codes: A61K 45/06 : Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61K 47/26 : Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
• CPC Class Codes: A61K 9/0034 : Urogenital system, e.g. vagina, uterus, cervix, penis, scrotum, urethra, bladder; Personal lubricants
• CPC Class Codes: A61P 15/08 : for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
• CPC Class Codes: C12Q 1/6883 : for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 2600/156 : Polymorphic or mutational markers

Abstract

A kit having instructions for use for performing uterine lavage in a female patient includes a uterine lavage catheter configured for insertion into a woman'"'"'s uterus to remove viable blastocysts from the uterus, and one or more first containers having a sufficient dosage amount of a GnRH antagonist to cause desynchronization of the endometrium of the patient prior to, during and/or following recovery of viable blastocysts from the uterus.

Claims

1. A kit for uterine lavage, consisting of:

a uterine lavage catheter configured for insertion into a woman'"'"'s uterus to remove blastocysts from the uterus; and

one or more first containers with a sufficient dosage amount of a GnRH antagonist to cause corpus luteum apoptosis leading to desynchronization of the endometrium of the uterus.

2. A kit for uterine lavage, consisting of:

a uterine lavage catheter configured for insertion into a woman'"'"'s uterus;

one or more first containers with a sufficient dosage amount of a GnRH antagonist to cause corpus luteum apoptosis leading to desynchronization of the endometrium of the uterus; and

a cell-culture container for collecting lavage fluid from the lavage catheter.

3. A kit for uterine lavage, consisting of:

a uterine lavage catheter configured for insertion into a woman'"'"'s uterus;

one or more first containers with a sufficient dosage amount of a GnRH antagonist to cause corpus luteum apoptosis leading to desynchronization of the endometrium of the uterus; and

a lavage controller for controlling delivery of pressure and application of vacuum to the lavage catheter.

4. A kit for uterine lavage, consisting of:

a uterine lavage catheter configured for insertion into a woman'"'"'s uterus;

one or more first containers with a sufficient dosage amount of a GnRH antagonist to cause corpus luteum apoptosis leading to desynchronization of the endometrium of the uterus; and

a programmable lavage controller for automatically controlling delivery of pressure and application of vacuum to the lavage catheter.

5. A kit for uterine lavage, consisting of:

a uterine lavage catheter configured for insertion into a woman'"'"'s uterus for removal of one or more blastocysts from the uterus;

one or more first containers with a sufficient dosage amount of a GnRH antagonist to cause corpus luteum apoptosis leading to desynchronization of the endometrium of the uterus; and

a cell transport vial for transport of lavage fluid containing the one or more blastocysts to a laboratory.

6. A kit for uterine lavage, consisting of:

a uterine lavage catheter configured for insertion into a woman'"'"'s uterus for removal of one or more blastocysts from the uterus;

one or more first containers with a sufficient dosage amount of a GnRH antagonist to cause corpus luteum apoptosis leading to desynchronization of the endometrium of the uterus; and

one or more of a;

(i) cell culture container for maintaining blastocyst-safe O2/CO2 levels without the requirement of an incubator, (ii) a cell transport vial for transport of lavage fluid containing the one or more blastocysts to a laboratory, (iii) a lavage controller for controlling delivery of pressure and application of vacuum to the lavage catheter, and (iv) a programmable controller for automatically controlling the delivery of pressure and the application of vacuum to the lavage catheter.