Fabrication of vascularized tissue using microfabricated two-dimensional molds
First Claim
1. A lamina assembly comprising:
- a lower structure having an upper side defining lower structure channels;
an upper structure having a lower side defining at least one upper structure microchannel that opposes the lower structure channels;
a semi-permeable membrane disposed between the lower and upper structures to separate the respective channels; and
cells cultured on at least one side of the semi-permeable membrane.
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Accused Products
Abstract
Methods and materials for making complex, living, vascularized tissues for organ and tissue replacement, especially complex and/or thick, structures, such as liver tissue is provided. Tissue lamina is made in a system comprising an apparatus having (a) a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, wherein the first and second molds or polymer scaffolds have means defining microchannels positioned toward the semi-permeable membrane, wherein the first and second molds or polymer scaffolds are fastened together; and (b) animal cells. Methods for producing complex, three-dimensional tissues or organs from tissue lamina are also provided.
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Citations
19 Claims
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1. A lamina assembly comprising:
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a lower structure having an upper side defining lower structure channels; an upper structure having a lower side defining at least one upper structure microchannel that opposes the lower structure channels; a semi-permeable membrane disposed between the lower and upper structures to separate the respective channels; and cells cultured on at least one side of the semi-permeable membrane. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A lamina assembly for supplementing or replacing at least one organ function, comprising:
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a lower structure having an upper side defining a plurality of lower microchannels; an upper structure having a lower side defining at least one channel that opposes at least a portion of the lower microchannels; a semi-permeable membrane disposed between the lower microchannels and the at least one channel; and cells cultured on at least one of the semi-permeable membrane, the lower structure or the upper structure. - View Dependent Claims (15, 16, 17, 18, 19)
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Specification