Disposable fluidic cartridge and components

• US 10,232,369 B2
• Filed: 03/24/2017
• Issued: 03/19/2019
• Est. Priority Date: 03/24/2016
• Status: Active Grant

• Current Assignee: Biological Dynamics, Inc.
• Original Assignee: Biological Dynamics, Inc.
• Inventors: Turner, Robert, Madsen, James, Yang, Kai, Hinestrosa Salazar, Juan Pablo, Krishnan, Raj, Simon Herrera, Pedro David
• Primary Examiner(s): Sines, Brian J.
• Application Number:
  US15/469,406
• Publication Number:
  US 20170274378A1
• Time in Patent Office: 725 Days
• Field of Search: 422 681, 422502, 422503, 422504, 436 43, 436180
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/023 Sending and receiving of information, e.g. using bluetooth
• CPC Class Codes: B01L 2300/027 Digital display, e.g. LCD, LED
• CPC Class Codes: B01L 2300/041 Connecting closures to device or container
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2300/165 Specific details about hydrophobic, oleophobic surfaces
• CPC Class Codes: B01L 2300/168 Specific optical properties, e.g. reflective coatings
• CPC Class Codes: B01L 2400/0424 Dielectrophoretic forces
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0638 membrane valves, flap valves
• CPC Class Codes: B01L 2400/0694 vents used to stop and induce flow, backpressure valves
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces

Abstract

Disclosed are cartridge components, cartridges, systems, and methods for isolating analytes from biological samples. In various aspects, the cartridge components, cartridges, systems, and methods may allow for a rapid procedure that requires a minimal amount of material from complex fluids.

Claims

1. A fluidic cartridge configured to close upon activation, the fluidic cartridge comprising:

a. at least one inlet comprising a first self-sealing polymer;

b. a sample reservoir;

c. a reagent reservoir;

d. at least one bubble trap reservoir configured to trap air bubbles;

e. a detection window configured for analysis of the biological sample; and

f. a waste reservoir, comprising at least one outlet comprising a second self-sealing polymer,

wherein the sample reservoir and the reagent reservoir each comprise a sealing, gas-impermeable, removable cover,

wherein the at least one inlet, reagent reservoir, sample reservoir, bubble trap reservoir, detection window, and waste reservoir are connected by a continuous fluidic channel, and

wherein the first self-sealing polymer and second self-sealing polymer are activated upon contact with liquid to form a liquid-tight seal in the fluidic cartridge.

2. The fluidic cartridge of claim 1, wherein liquids in the sample reservoir and the reagent reservoir stay within the sample reservoir or the reagent reservoir until pressure is applied to the inlet.

3. The fluidic cartridge of claim 1, wherein the at least one inlet and the at least one outlet each further comprise:

a port and a filter.

4. The fluidic cartridge of claim 3, wherein the port is an opening smaller than the inlet or outlet and the filter is a porous polyurethane filter.

5. The fluidic cartridge of claim 1, wherein the self-sealing polymer comprises a hydrophilic polyurethane, a hydrophilic polyurea, or a hydrophilic polyureaurethane.

6. The fluidic cartridge of claim 1, wherein the bubble trap reservoir is positioned downstream of and fluidly connected to the sample reservoir and the reagent reservoir by a continuous fluidic channel.

7. The fluidic cartridge of claim 1, further comprising two or more bubble trap reservoirs.

8. The fluidic cartridge of claim 7, wherein the two or more bubble trap reservoirs are sequentially connected by the continuous fluidic channel.

9. The fluidic cartridge of claim 1, wherein the size of the cross sectional area of the fluidic channel going into and out of the sample reservoir and the fluidic channel going into and out of the reagent reservoir provides sufficient fluidic resistance to prevent fluid in the sample reservoir or the reagent reservoir from leaving the reservoir without pressure applied to the inlet.

10. The fluidic cartridge of claim 1, wherein the bubble trap has a cross sectional area that is at least two times larger than a cross sectional area of the fluidic channel.

11. The fluidic cartridge of claim 1, wherein the fluidic channel has a cross sectional area of about 0.25 mm² and the bubble trap has a cross sectional area of about 8 mm².

12. The fluidic cartridge of claim 1, wherein the bubble trap has a length of at least 3 mm, a width of least 3 mm, and a height of least 1 mm.

13. The fluidic cartridge of claim 1, wherein the bubble trap has a length of at least 3 mm, a width of at least 5 mm, and a height of at least 1 mm.

14. The fluidic cartridge of claim 1, wherein the bubble trap has a maximum length of 7 mm, a maximum width of 10 mm, and a maximum height of 5 mm.

15. The fluidic cartridge of claim 1, wherein the bubble trap has a maximum length of 5 mm, a maximum width of 8 mm, and a maximum height of 3 mm.

16. The fluidic cartridge of claim 1, wherein the bubble trap is a cylinder or sphere, the cylinder or sphere having a diameter of at least 3 mm.

17. The fluidic cartridge of claim 1, wherein the bubble trap is a cylinder or sphere, the cylinder or sphere having a diameter of at least 5 mm.

18. A compact device for isolating nanoscale analytes in a sample, the compact device comprising:

a. a housing;

b. an optical pathway;

c. a fluid-moving mechanism;

d. an electronic chip; and

e. the fluidic cartridge system of claim 1;

wherein the compact device is controlled by a mobile computing device and the compact device has a power requirement of no more than 5 Watts.

19. The compact device of claim 18, wherein the analyte in the sample is detected with a camera on the mobile computing device and the camera produces an image that is analyzed by the mobile computing device.

20. The compact device of claim 18, wherein the fluid-moving mechanism comprises a pump, wherein the pump is a syringe, a peristaltic pump, or a piezo pump.

21. The compact device of claim 18, wherein the electronic chip is configured to control the fluidic cartridge and to apply an electric current to the sample.

22. The compact device of claim 18, wherein the fluidic cartridge further comprises a plurality of alternating current (AC) electrodes configured to be selectively energized to establish dielectrophoretic (DEP) high field and dielectrophoretic low field regions, wherein AC electrokinetic effects separate nanoscale analytes from larger molecular entities.

23. The compact device of claim 18, wherein the fluidic cartridge is inserted into a fluidic cartridge slot of the compact device.

24. A system for detecting analytes or other microparticulates in a sample, the system comprising:

a. a compact device comprising;

a housing, an optical pathway, a fluid-moving mechanism, and an electrical chip;

b. a mobile computing device comprising;

at least one processor, a memory, and an operating system configured to perform executable instructions; and

c. the fluidic cartridge of claim 1,

wherein the compact device is configured to receive a mobile computing device and a fluidic cartridge; and

wherein the compact device positions the mobile computing device and the fluidic cartridge relative to each other to detect analytes or other microparticulates in the sample.

25. The system of claim 24, wherein the mobile computing device is a smart phone, a tablet computer, or a laptop computer.

26. The system of claim 24, wherein the mobile computing device comprises a connection port that connects to the compact device via a charging port, a USB port, or a headphone port of the mobile computing device.

27. The system of claim 24, wherein the compact device is powered by the mobile computing device, a battery, a solar panel, or a wall outlet.

28. The system of claim 24, wherein the analyte or other microparticulates in the sample are detected with a camera on the mobile computing device.