Capillary barriers for staged loading of microfluidic devices

• US 10,233,441 B2
• Filed: 12/22/2017
• Issued: 03/19/2019
• Est. Priority Date: 03/14/2013
• Status: Active Grant

• Current Assignee: Board of Trustees of the Leland Stanford Junior University (Stanford Management Co.)
• Original Assignee: Board of Trustees of the Leland Stanford Junior University (Stanford Management Co.)
• Inventors: Santiago, Juan G., Marshall, Lewis A., Rogacs, Anita
• Primary Examiner(s): Xu, Xiaoyun R
• Application Number:
  US15/853,370
• Publication Number:
  US 20180237767A1
• Time in Patent Office: 452 Days
• Field of Search: None
• CPC Class Codes: B01D 57/02 by electrophoresis treatment of water, waste water, sewage or sludge by electrophoresis C02F1/469; electrophoretic production of compounds or non-metals C25B7/00; investigating or analysing materials by using electrophoresis G01N27/26
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2200/0631 Purification arrangements, e.g. solid phase extraction [SPE]
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/0838 Capillaries
• CPC Class Codes: B01L 2300/0858 Side walls
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/12 Specific details about materials
• CPC Class Codes: B01L 2300/165 Specific details about hydrophobic, oleophobic surfaces
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 2400/0688 surface tension valves, capillary stop, capillary break
• CPC Class Codes: B01L 2400/086 using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 2400/088 by specific surface properties
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: C12N 15/101 by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
• CPC Class Codes: C12Q 1/6806 : Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: G01N 27/44791 : Microapparatus sample containers with integrated microfluidic structures B01L3/5027

Abstract

Various aspects of the present disclosure are directed toward methods and apparatuses for interacting a first liquid and a second liquid in one or more fluidic channels of a capillary structure. The methods and apparatuses can include providing at least one capillary barrier that positions a meniscus of the first liquid at a fluid-interface region using capillary forces within the capillary structure. Additionally, a path is provided along one of the channels for the second liquid to flow toward the fluid-interface region. Additionally, gas pressure is released, via a gas-outflow port, from the fluid-interface region while flow of the first liquid is arrested. Further, the first liquid and the second liquid contact in the fluid-interface region with the capillary barrier holding the first liquid at the fluid-interface region.

Claims

1. An apparatus comprising:

a fluidic system including a first microfluidic channel configured and arranged to guide a first liquid toward a fluid-interface region and including a second microfluidic channel configured and arranged to guide a second liquid toward the fluid-interface region;

a capillary barrier configured and arranged to arrest flow of the first liquid at the fluid-interface region using capillary forces, wherein the capillary barrier is at least in part defined by a change in a cross-sectional area, the change in the cross-sectional area being sufficient to arrest flow of the first liquid at the fluid-interface region;

a second capillary barrier configured and arranged to arrest flow of the second liquid at the fluid-interface region using capillary forces thereby to preserve an interface between the first liquid and the second liquid; and

an escape path through which fluid can escape from the fluid-interface region.

2. The apparatus of claim 1, wherein the first liquid and the second liquid are loaded without using a vacuum.

3. The apparatus of claim 1, wherein the first microfluidic channel and the second microfluidic channel are formed from a polymeric material.

4. The apparatus of claim 1, wherein the first microfluidic channel and the second microfluidic channel respectively include hydrophobic surfaces.

5. The apparatus of claim 1, further including:

a microfluidic chip configured to interface or interact with fluid, and

circuitry configured and arranged to receive data indicative of an interaction between the first liquid and the second liquid and to perform microfluidics analysis of quantification and quality control of one or more of DNA, RNA, proteins, and cells in at least one of the first liquid and the second liquid.

6. The apparatus of claim 1, further including;

a microfluidic chip configured to interface or interact with fluid; and

circuitry configured and arranged to receive data indicative of an interaction between the first liquid and the second liquid, and to perform analysis of electrophoresis, analysis of separation, or analysis of extraction of DNA fragments in at least one of the first liquid and the second liquid.

7. The apparatus of claim 1, further including:

a microfluidic chip configured to interface or interact with fluid; and

circuitry configured and arranged to receive data indicative of an interaction between the first liquid and the second liquid, and to perforin analysis of enrichment of target nucleic acid in at least one of the first liquid and the second liquid.

8. The apparatus of claim 1, further including a gas-outflow port configured and arranged to release gas pressure while flow of the first liquid is arrested, and wherein during operation, the second capillary barrier arrests flow of the second liquid at the fluid-interface region and a liquid-liquid interface is formed.

9. The apparatus of claim 8, wherein the gas-outflow port is configured and arranged to permit release of air while flow of the first liquid is arrested.

10. The apparatus of claim 1, wherein the escape path includes a region for limiting a volume flow rate or a volume of the fluid;

and the escape path is connected to a gas-outflow port configured and arranged to release gas pressure while flow of the first liquid is arrested.

11. The apparatus of claim 10, wherein the region for limiting a volume flow rate or a volume of the fluid includes an expansion of the cross-sectional area.

12. The apparatus of claim 1, wherein the change of the cross-sectional area is an expansion of the cross-sectional area.

13. The apparatus of claim 1, wherein the capillary barrier includes a protrusion, ramp, or post included on a wall of the first microfluidic channel upstream of the fluid-interface region.

14. The apparatus of claim 1, wherein the escape path includes a low volume channel, a constricted channel, a protrusion, a ramp or a post.

15. The apparatus of claim 1, wherein the escape path includes a region of the capillary barrier is configured and arranged to decrease dimensions of the cross-sectional area of the escape path.

16. The apparatus of claim 1, wherein the fluid escapes from the fluid-interface region and through the escape path is a gas.

17. The apparatus of claim 1, wherein the escape path further comprises the second capillary barrier, and wherein the second capillary barrier is further configured and arranged to arrest flow of the second liquid at the fluid-interface region, wherein an interface between the first liquid and the second liquid results and a meniscus of the second liquid is maintained.

18. The apparatus of claim 1, wherein the escape path is configured and arranged to limit fluid flow due to increased hydraulic resistance in the escape path.

19. The apparatus of claim 1, wherein the second capillary barrier is further configured and arranged to arrest flow of the second liquid at the fluid-interface and to fat n a stationary interface or front where the first liquid meets the second liquid.

20. A method for interacting a first liquid and a second liquid in one or more fluidic channels, the method comprising:

providing a capillary barrier to position a meniscus of the first liquid at a fluid-interface region using capillary forces;

providing a path along one of the channels for the second liquid to flow toward the fluid-interface region;

providing a second capillary barrier to position a meniscus of the second liquid at the fluid-interface region;

providing an escape path for escaping fluid to exit from the region between the first and second liquid;

limiting a volume flow rate or a volume of the escaping fluid;

arresting flow of the second liquid at the fluid-interface region with the second capillary barrier; and

causing the first liquid and the second liquid to contact in the fluid-interface region thereby preserving an interface between the first liquid and the second liquid.

21. The method of claim 20, further including a gas-outflow port connected to the escape path, wherein the first liquid and the second liquid merge in the fluid-interface region.

22. A method for interacting a first liquid and a second liquid in one or more fluidic channels, the method comprising:

providing a capillary barrier to position a meniscus of the first liquid at a fluid-interface region using capillary forces;

providing a path along one of the channels for the second liquid to flow toward the fluid-interface region;

providing a second capillary barrier to position a meniscus of the second liquid at the fluid-interface region;

using the second capillary barrier and capillary forces to arrest flow of the second liquid at the fluid-interface region; and

causing the first liquid and the second liquid to contact in the fluid-interface region.

23. The method of claim 22, further including providing an escape path for escaping fluid to exit from the region between the first and second liquid, and limiting a volume flow rate or a volume of the escaping fluid.