Method for optically examining small amounts of liquid using a cuvette and insert parts that form a column of liquid between two measuring areas
First Claim
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1. A method for optically examining small amounts of liquid, the method comprising the steps of:
- providing a cuvette, comprising;
insert parts with two measuring areas and an adapter for insertion into a cuvette shaft of an optical measuring device and means for detachably holding the insert parts on the adapter, the measuring areas being in a distance from each other, for positioning a liquid sample between the measuring areas in a beam path of the optical measuring device that crosses the cuvette shaft, wherein the insert parts and adapter are fixedly connected to each other, and wherein the cuvette shaft having a bottom area of 12.5×
12.5 mm;
positioning a volume of a liquid sample in the range of 0.2 to 5 microliters between the measuring areas, the liquid sample is placed with the aid of a pipette on one of the two measuring areas;
forming a column of liquid between the two measuring areas by the surface tension of the liquid, through which an optical measurement can be performed; and
inserting the adapter in the cuvette shaft with the sample in the beam path of the optical measuring device.
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Abstract
Cuvette, comprising at least one measuring area on each one of two arms that are pivotally connected to each other such that from a swung-apart condition, they can be swung together into a measuring position in which the two measuring areas have a distance for positioning a sample between the measuring areas, and means for positioning the two arms in a measuring position in a cuvette shaft of an optical measuring device with a sample between the two measuring areas in a beam path of the optical measuring device that crosses the cuvette shaft.
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Citations
29 Claims
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1. A method for optically examining small amounts of liquid, the method comprising the steps of:
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providing a cuvette, comprising;
insert parts with two measuring areas and an adapter for insertion into a cuvette shaft of an optical measuring device and means for detachably holding the insert parts on the adapter, the measuring areas being in a distance from each other, for positioning a liquid sample between the measuring areas in a beam path of the optical measuring device that crosses the cuvette shaft, wherein the insert parts and adapter are fixedly connected to each other, and wherein the cuvette shaft having a bottom area of 12.5×
12.5 mm;positioning a volume of a liquid sample in the range of 0.2 to 5 microliters between the measuring areas, the liquid sample is placed with the aid of a pipette on one of the two measuring areas; forming a column of liquid between the two measuring areas by the surface tension of the liquid, through which an optical measurement can be performed; and inserting the adapter in the cuvette shaft with the sample in the beam path of the optical measuring device. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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27. A method for optically examining small amounts of liquid, the method comprising the steps of:
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providing a cuvette, comprising;
insert parts with two measuring areas and an adapter for insertion into a cuvette shaft of an optical measuring device and means for detachably holding the insert parts on the adapter, the measuring areas being in a distance from each other, for positioning a liquid sample between the measuring areas in a beam path of the optical measuring device that crosses the cuvette shaft, wherein the insert parts and adapter are fixedly connected to each other, and wherein the cuvette shaft having a bottom area of 12.5×
12.5 mm;positioning a volume of a liquid sample in the range of 0.2 to 5 microliters between the measuring areas, wherein two drops of liquid are applied to two measuring areas, the drops are brought into contact with each other by drawing the two measuring areas near to each other, so that the drops coalesce into one single drop, and the one single drop is subjected to an optical measurement, and wherein a pipette is used for positioning the liquid sample on both measuring areas; and inserting the adapter in the cuvette shaft with the sample in the beam path of the optical measuring device.
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28. A method for optically examining small amounts of liquid, the method comprising the steps of:
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providing a cuvette, comprising;
insert parts with two measuring areas and an adapter for insertion into a cuvette shaft of an optical measuring device and means for detachably holding the insert parts on the adapter, the measuring areas being in a distance from each other, for positioning a liquid sample between the measuring areas in a beam path of the optical measuring device that crosses the cuvette shaft, wherein the insert parts and adapter are fixedly connected to each other, and wherein the cuvette shaft having a bottom area of 12.5×
12.5 mm;positioning a volume of a liquid sample in the range of 0.2 to 5 microliters between the measuring areas, the liquid sample is placed with the aid of a pipette on one of the two measuring areas; forming a column of liquid between the two measuring areas by the surface tension of the liquid, through which an optical measurement can be performed; wherein the layer thickness of a layer between the two measuring areas is ensured by magnetic forces from magnets, whose unlike poles are arranged in a short distance from each other when the device is closed, the magnets being integrated into a device components of the cuvette, which accommodate the inserts; and inserting the adapter in the cuvette shaft with the sample in the beam path of the optical measuring device. - View Dependent Claims (29)
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Specification