Linking sequence reads using paired code tags
First Claim
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1. An artificial transposon, comprising:
- a first transposase recognition site,a second transposase recognition site,a barcode disposed therebetween, wherein the barcode comprises a double-stranded nucleic acid sequence comprisinga first strand barcode anda second strand barcode, wherein the first strand barcode and the second strand barcode comprise non-complementary sequences; and
a fragmentation site disposed within the first strand barcode and the second strand barcode.
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Abstract
Artificial transposon sequences having code tags and target nucleic acids containing such sequences. Methods for making artificial transposons and for using their properties to analyze target nucleic acids.
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Citations
10 Claims
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1. An artificial transposon, comprising:
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a first transposase recognition site, a second transposase recognition site, a barcode disposed therebetween, wherein the barcode comprises a double-stranded nucleic acid sequence comprising a first strand barcode and a second strand barcode, wherein the first strand barcode and the second strand barcode comprise non-complementary sequences; and a fragmentation site disposed within the first strand barcode and the second strand barcode. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A DNA library preparation kit, comprising:
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a plurality of artificial transposons, wherein each artificial transposon of the plurality of artificial transposon comprises; a first transposase recognition site; a second transposase recognition site; a barcode disposed therebetween, wherein the barcode comprises a double-stranded nucleic acid sequence comprising a first strand barcode and a second strand barcode, wherein the first strand barcode and the second strand barcode comprise non-complementary sequences; and a fragmentation site disposed within the first strand barcode and the second strand barcode; and wherein each barcode of each artificial transposon of the plurality of artificial transposons is distinguishable from one another. - View Dependent Claims (10)
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Specification