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Preserving genomic connectivity information in fragmented genomic DNA samples

  • US 10,246,746 B2
  • Filed: 12/16/2014
  • Issued: 04/02/2019
  • Est. Priority Date: 12/20/2013
  • Status: Active Grant
First Claim
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1. A method of sequencing a target nucleic acid polymer, comprising:

  • (a) modifying a target nucleic acid polymer to produce a modified nucleic acid polymer, wherein the modified nucleic acid polymer comprises a plurality of sequence regions from the target nucleic acid polymer;

    (b) releasing fragments of the modified nucleic acid polymer onto a solid support surface, each fragment comprising one of the sequence regions;

    (c) capturing the fragments randomly at locations in an area of the solid support surface;

    (d) determining nucleotide sequences of the sequence regions by detecting the fragments at the locations;

    (e) determining relative physical distances between the fragments at the locations; and

    (f) producing a representation of the nucleotide sequence for the target nucleic acid polymer based on the nucleotide sequences from the fragments and the determined relative physical distances between the locations on the solid support surface, wherein the relative physical distances are indicative of distances between the sequence regions along a given strand of the target nucleic acid polymer.

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