Portable water quality instrument
First Claim
1. A method of measuring the amount of an analyte in water, the method comprising the steps of:
- obtaining a sample of water containing an analyte;
obtaining a calibration solution, wherein the calibration solution comprises an isotopic calibration standard;
producing a mixture of the sample, the calibration solution, and Raman-scattering nanoparticles;
illuminating the mixture with an electric field to produce Raman-spectra of the analyte and the isotopic calibration standard simultaneously; and
,performing a ratiometric analysis of the Raman-spectra of the anlayte and the isotopic calibration standard to quantify the analyte,wherein the analyte is selenate and the isotopic calibration standard is a sulfate isotope or a selenate isotope.
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Accused Products
Abstract
A hand-held microfluidic testing device is provided that includes a housing having a cartridge receiving port, a cartridge for input to the cartridge receiving port having a sample input and a channel, where the channel includes a mixture of Raman-scattering nanoparticles and a calibration solution, where the calibration solution includes chemical compounds capable of interacting with a sample under test input to the cartridge and the Raman-scattering nanoparticles, and an optical detection system in the housing, where the optical detection system is capable of providing an illuminated electric field, where the illuminating electric field is capable of being used for Raman spectroscopy with the Raman-scattering nanoparticles and the calibration solution to analyze the sample under test input to the cartridge.
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Citations
27 Claims
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1. A method of measuring the amount of an analyte in water, the method comprising the steps of:
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obtaining a sample of water containing an analyte; obtaining a calibration solution, wherein the calibration solution comprises an isotopic calibration standard; producing a mixture of the sample, the calibration solution, and Raman-scattering nanoparticles; illuminating the mixture with an electric field to produce Raman-spectra of the analyte and the isotopic calibration standard simultaneously; and
,performing a ratiometric analysis of the Raman-spectra of the anlayte and the isotopic calibration standard to quantify the analyte, wherein the analyte is selenate and the isotopic calibration standard is a sulfate isotope or a selenate isotope. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A portable testing device comprising:
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a portable testing device housing, wherein said portable testing device housing comprises a cartridge receiving port; a cartridge for input to said cartridge receiving port, wherein said cartridge comprises a mixture of a water sample comprising an analyte under investigation, metallic nanoparticles and a calibration solution, wherein said calibration solution comprises an isotopic calibration standard of the analyte under investigation; and an optical detection system in said portable testing device housing, wherein said optical detection system is configured to provide an illuminating electric field, wherein said illuminating electric field is configured for Raman spectroscopy with said mixture, wherein said cartridge and said optical detection system are configured to produce analyte and isotopic calibration standard simultaneously and the cartridge comprises the isotopic calibration standard in an amount sufficient to allow for a ratiometric analysis of the simultaneously produced spectra to quantify the analyte, wherein the isotopic calibration standard is an isotope of selenate or sulfate. - View Dependent Claims (11)
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12. A portable testing device comprising:
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a. a portable testing device housing, wherein said portable testing device housing comprises a cartridge receiving port; b. a cartridge for input to said cartridge receiving port, wherein said cartridge comprises a channel, wherein said channel comprises a mixture of a water sample comprising an analyte under investigation, metallic Raman-scattering nanoparticles and a calibration solution, wherein said calibration solution comprises an isotope of the analyte under investigation, wherein said isotope comprises a first number of neutrons and said analyte comprises a second number of neutrons, wherein said isotope differs from said analyte only by the number of said neutrons; and c. an optical detection system in said portable testing device housing, wherein said optical detection system is configured to provide an illuminating electric field, wherein said illuminating electric field is configured for Raman spectroscopy with said metallic Raman-scattering nanoparticles and mixture, and wherein said cartridge and said optical detection system are configured to produce Raman spectra of the analyte and isotope simultaneously, wherein the analyte is selenate and the isotope is an isotope of selanate or sulfate. - View Dependent Claims (13, 14, 15)
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16. A method of testing a water sample to determine a concentration of an analyte in the water sample comprising the steps of,
collecting a water sample containing an analyte at a site; -
adding metallic Raman-scattering nanoparticles, an isotopic reference standard and an amine or thiol to the sample to produce a mixture; providing a portable testing device comprising, (i) a portable testing device housing, wherein said portable testing device housing comprises a cartridge receiving port;
(ii) a cartridge for input to said cartridge receiving port, wherein said cartridge contains the mixture;and (iii) an optical detection system in said portable testing device housing, providing an illuminating electric field from said optical detection system thereby producing Raman spectra of the analyte and isotope simultaneously while the cartridge is input to said cartridge receiving port; and performing a ratiometric analysis of the simultaneously produced analyte and isotope spectra to quantify the analyte originally present in the water sample, wherein the analyte under investigation is selenate and the isotopic calibration standard comprises a selenate isotope or a sulfate isotope. - View Dependent Claims (17)
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18. A portable testing device comprising,
a portable testing device housing, wherein said portable testing device housing comprises a cartridge receiving port; -
a cartridge for input to said cartridge receiving port, wherein said cartridge contains a mixture including a water sample comprising selenate, metallic Raman-scattering nanoparticles, and a selenate isotope or a sulfate isotope; and an optical detection system in said portable testing device housing, wherein said optical detection system is configured to provide an illuminating electric field, wherein said illuminating electric field is configured to produce Raman spectra of the selenate and isotope simultaneously while the cartridge is input to said cartridge receiving port to allow for a ratiometric analysis of the simultaneously produced selenate and isotope spectra to quantify the selenate in the water sample. - View Dependent Claims (19, 20, 21, 22)
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23. A method for determining the concentration of selenate in a water sample comprising the steps of,
adding metallic Raman-scattering nanoparticles to the water sample; -
adding a selenate isotope or a sulfate isotope to the water sample; applying an illuminating electric field to the water sample thereby producing Raman spectra of the selenate and isotope simultaneously; and
,analysing the simultaneously produced selenate and isotope spectra to quantify the selenate in the water sample. - View Dependent Claims (24, 25, 26, 27)
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Specification