Lighting systems and methods of using lighting systems for in virto potency assay for photofrin

• US 10,247,723 B2
• Filed: 06/06/2016
• Issued: 04/02/2019
• Est. Priority Date: 06/01/2012
• Status: Active Grant

• Current Assignee: Concordia Laboratories Inc. (ADVANZ PHARMA Corp. Ltd.)
• Original Assignee: Concordia Laboratories Inc. (ADVANZ PHARMA Corp. Ltd.)
• Inventors: Roberts, Michael Scot
• Primary Examiner(s): Mai, Anh T
• Assistant Examiner(s): Fallahkhair, Arman B
• Application Number:
  US15/175,013
• Publication Number:
  US 20160282332A1
• Time in Patent Office: 1,030 Days
• CPC Class Codes: A61K 41/00 Medicinal preparations obtained by treating materials with wave energy or particle radiation ; Therapies using these preparations
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
• CPC Class Codes: C12Q 1/22 Testing for sterility conditions
• CPC Class Codes: F21V 13/14 the elements being filters or photoluminescent elements, reflectors and refractors
• CPC Class Codes: F21V 5/04 of lens shape
• CPC Class Codes: F21V 7/00 Reflectors for light sources characterised by cooling arrangements F21V29/505
• CPC Class Codes: F21V 7/22 characterised by materials, surface treatments or coatings, e.g. dichroic reflectors
• CPC Class Codes: F21V 9/04 for filtering out infrared radiation dichroic reflectors F21V7/22; using liquid-filled chambers F21V9/12
• CPC Class Codes: G01N 21/255 Details, e.g. use of specially adapted sources, lighting or optical systems
• CPC Class Codes: G01N 2201/061 Sources
• CPC Class Codes: G01N 2201/0633 Directed, collimated illumination
• CPC Class Codes: G01N 2201/0686 Cold filter; IR filter
• CPC Class Codes: G01N 33/5011 for testing antineoplastic activity
• CPC Class Codes: G02B 21/06 Means for illuminating specimens
• CPC Class Codes: G02B 3/08 with discontinuous faces, e.g. Fresnel lens diffractive Fresnel lenses G02B5/1876

Abstract

Presently disclosed is a lighting system and methods of using the lighting system for in vitro potency assay for photofrin. The lighting system includes a lamp housing, a first lens, an infrared absorbing filter, an optical filter, and a second lens. The lamp housing includes a lamp and a light-port. In operation, broad spectrum light from the lamp exits the lamp housing by passing through the light-port. The first lens then collimates the broad spectrum light that exits the lamp housing through the light-port. The infrared absorbing filter then passes a first portion of the collimated broad spectrum light to the optical filter and absorbs infrared light of the broad spectrum light. The optical filter then passes a second portion of the collimated broad spectrum light to the second lens. The second lens then disperses the second portion of the collimated light to provide uniform irradiation of a cell culture plate. A method of using the lighting system for studying a photosensitizer is also disclosed.

Claims

1. A lighting system comprising:

a lamp housing including a lamp and a light-port, wherein broad spectrum light from the lamp exits the lamp housing through the light-port;

a first lens to collimate the broad spectrum light that exits the lamp housing through the light-port;

an infrared absorbing filter to pass a first portion of the collimated broad spectrum light and absorb infrared light of the broad spectrum light that passes through the light-port and that is collimated by the first lens;

a reflector to reflect the first portion of the collimated broad spectrum light that passes through the infrared absorbing filter;

a short pass filter to pass a second portion of the collimated broad spectrum light, wherein the second portion of the collimated broad spectrum light comprises a subset of the first portion of the collimated broad spectrum light, and wherein the short pass filter filters out light of the first portion of the collimated broad spectrum light of wavelengths greater than 650 nm and less than 595 nm;

an infrared blocking filter to pass the second portion of the collimated broad spectrum light after passing through the short pass filter, wherein the infrared blocking filter absorbs residual infrared light of the first portion of the collimated broad spectrum light; and

a second lens to disperse the second portion of the collimated light that passed through the infrared blocking filter and the short pass filter.

2. A method for studying a photosensitizer, the method comprising:

adding the photosensitizer to a portion of wells on a cell culture plate to form photosensitizer assay wells, the wells comprising carcinoma cells;

incubating the photosensitizer assay wells for a first predetermined time period;

optionally washing the photosensitizer assay wells;

irradiating the photosensitizer assay wells with the lighting system of claim 1 at a predetermined wavelength to form irradiated wells, wherein each well is uniformly irradiated;

incubating the irradiated wells for a second predetermined time period; and

determining percent viability of the carcinoma cells contained in the wells.

3. The method according to claim 2, wherein the photosensitizer is a porphyrin-based anti-neoplastic agent.

4. The method according to claim 3, wherein the porphyrin-based anti-neoplastic agent is porfimer sodium.

5. The method according to claim 2, wherein the photosensitizer assay wells are irradiated with light within a range of 400 nm to 650 nm.

6. The method according to claim 2, wherein the carcinoma cells are A549 human lung carcinoma cells.

7. The method according to claim 2, wherein the cell culture plate is an opaque black plate.

8. The lighting system of claim 1, further comprising:

a base positioned on a surface below the second lens;

a ring stand coupled to the base; and

a first support ring removably attached to the ring stand.

9. The lighting system of claim 8,

wherein the second lens comprises a first dispersing lens and a second dispersing lens;

and

wherein the first support ring holds the first dispersing lens and the second dispersing lens in place.

10. The lighting system of claim 9,

wherein the first dispersing lens comprises a first plano convex lens;

wherein the second dispersing lens comprises a second plano convex lens;

wherein the first plano convex lens comprises a first plano side and a first convex side;

wherein the second plano convex lens comprises a second plano side and a second convex side; and

wherein the first convex side is adjacent to the second convex side with a gap between the first convex side and the second convex side.

11. The lighting system of claim 1, further comprising:

a wall,

wherein the light-port is located within the wall, and

wherein a position of the second lens is adjustable in at least one of a direction parallel to the wall and a direction perpendicular to the wall.

12. The lighting system of claim 11, further comprising:

a shelf, and

a lens slider including a first hole for passing light;

wherein the shelf comprises a shelf riser parallel to the wall,

wherein the shelf comprises a shelf top perpendicular to the wall,

wherein the shelf top includes a second hole for passing light,

wherein the second lens is removably attached to the lens slider,

wherein the lens slider is removably attached to the shelf top, and

wherein at least a portion of the first hole is above or below at least a portion of the second hole.

13. A method comprising:

using the lighting system of claim 1 to irradiate contents of a cell culture plate.

14. The lighting system of claim 1, wherein the first lens comprises a condenser lens or a Fresnel lens.

15. The lighting system of claim 1, wherein the reflector comprises a dichroic mirror.

16. The lighting system of claim 1, wherein the lamp housing is sealed so that less than 1% of the broad spectrum light from the lamp exits the lamp housing other than through the light-port.

17. The lighting system of claim 1,

wherein the lamp housing comprises a top,

wherein the light-port is within the top,

wherein the first lens is within the light-port, and

wherein the infrared absorbing filter, the infrared blocking filter, the short pass filter, and the second lens are located at positions above the top.

18. The lighting system of claim 1,

wherein the lamp housing comprises a bottom,

wherein the light-port is within the bottom,

wherein the first lens is within the light-port, and

wherein the infrared absorbing filter, the infrared blocking filter, the short pass filter, and the second lens are located at positions below the bottom.

19. The lighting system of claim 1, wherein the collimated broad spectrum light has a spectral range of 400 nm to 700 nm, and wherein the second portion of the collimated broad spectrum light has a spectral range of 595 nm to 645 nm.