Method and assay for detection of residues

• US 10,247,728 B1
• Filed: 03/16/2017
• Issued: 04/02/2019
• Est. Priority Date: 03/17/2016
• Status: Active Grant

• Current Assignee: Charm Sciences, Inc.
• Original Assignee: Charm Sciences, Inc.
• Inventors: Markovsky, Robert J., Douglas, David W.
• Primary Examiner(s): Brown, Melanie
• Application Number:
  US15/460,594
• Time in Patent Office: 747 Days
• CPC Class Codes: G01N 2333/36 from Actinomyces; from Streptomyces (G)
• CPC Class Codes: G01N 2469/20 Detection of antibodies in sample from host which are directed against antigens from microorganisms
• CPC Class Codes: G01N 33/54388 based on lateral flow
• CPC Class Codes: G01N 33/558 using diffusion or migration of antigen or antibody immunochromatographic test strips G01N33/54387
• CPC Class Codes: G01N 33/56938 Staphylococcus
• CPC Class Codes: G01N 33/9446 Antibacterials

Abstract

Embodiments described herein include detecting an analyte in a low pH sample. Some embodiments include detection of multiple analytes in a sample utilizing a plurality of analyte binders and a control zone containing multiple control zone capture agents. In some embodiments, the multiple control zone capture agents capture a plurality of binders within one control zone.

Claims

1. A lateral flow assay system for detecting one or more analytes in a solution, the system comprising:

(a) a buffer comprising a carrier protein and a base configured to increase the pH of a low pH sample from a pH between about 2 and about 5 to an optimal pH of about 7, wherein the combination of the low pH sample and the buffer forms the solution;

(b) a binder configured for generating a detectable signal, wherein the binder being adapted to combine with an analyte from the solution to form a binder-analyte complex; and

(c) a test strip configured to allow a test solution to flow, wherein the test solution contains either or both a binder and a binder-analyte complex, the test strip comprising;

(i) a test zone having immobilized thereon a test zone capture agent that, when the binder has not formed a binder-analyte complex, captures the binder,

(ii) a control zone having at least one control zone capture agent adapted to capture the binder, whether or not the binder has formed a binder-analyte complex, and

wherein capture of the binder at either the test zone or control zone results in a detectable signal and wherein a greater signal in the control zone as compared to the test zone indicates a positive result.

2. The system of claim 1, wherein the low pH sample has a pH of about 3.

3. The system of claim 1, wherein the low pH sample is a citrus juice.

4. The system of claim 3, wherein the citrus juice is selected from the group consisting of lemon juice, lime juice, grapefruit juice, and orange juice.

5. The system of claim 1, wherein the carrier protein is bovine serum albumin.

6. The system of claim 1, wherein the base is sodium bicarbonate.

7. The system of claim 6, wherein the sodium bicarbonate is about 0.7M.

8. The system of claim 1, wherein the analyte includes an antibiotic.

9. The system of claim 8, wherein the antibiotic comprises oxytetracycline.

10. The system of claim 8, wherein the antibiotic comprises streptomycin.