Flow cell alignment methods and systems

• US 10,249,038 B2
• Filed: 01/23/2017
• Issued: 04/02/2019
• Est. Priority Date: 03/15/2013
• Status: Active Grant

• Current Assignee: QIAGEN Sciences, LLC (Qiagen NV)
• Original Assignee: QIAGEN Sciences, LLC (Qiagen NV)
• Inventors: Stoops, David Ray, Veatch, Phillip Alan, Gordon, Steven Jeffrey
• Primary Examiner(s): Haque, Md N
• Assistant Examiner(s): Slater, Alison
• Application Number:
  US15/412,743
• Publication Number:
  US 20170132787A1
• Time in Patent Office: 799 Days
• CPC Class Codes: G01N 15/14 Optical investigation techniques, e.g. flow cytometry
• CPC Class Codes: G01N 2015/0038 Investigating nanoparticles
• CPC Class Codes: G01N 2015/144 Imaging characterised by its optical setup
• CPC Class Codes: G02B 21/10 affording dark-field illumination G02B21/14 and G02B21/125 take precedence
• CPC Class Codes: G02B 21/16 adapted for ultraviolet illumination ; Fluorescence microscopes G02B21/0076 takes precedence
• CPC Class Codes: G02B 21/365 Control or image processing arrangements for digital or video microscopes G02B21/361, G02B21/362 take precedence
• CPC Class Codes: G06T 2200/32 involving image mosaicing
• CPC Class Codes: G06T 2207/10056 Microscopic image
• CPC Class Codes: G06T 2207/30024 Cell structures in vitro; Tissue sections in vitro
• CPC Class Codes: G06T 7/0012 Biomedical image inspection
• CPC Class Codes: G06T 7/73 using feature-based methods
• CPC Class Codes: H04N 7/18 Closed-circuit television [CCTV] systems, i.e. systems in which the video signal is not broadcast

Abstract

A DNA flow cell processing method including positioning the flow cell on a stage at a predetermined location relative to a camera, illuminating the flow cell from a side with a first light source to reflect light off the DNA fragment bead locations, obtaining a first image of the flow cell and identifying a first reference pattern of bead locations in the first image, moving at least one of the flow cell and the stage relative to the camera, attempting to reposition the stage at the predetermined location, obtaining a second image of the flow cell, identifying the first reference pattern in the second image, and evaluating a first offset, relative to the camera, between the first reference pattern in the first image and the first reference pattern in the second image.

Claims

1. A method for processing flow cells containing deoxyribonucleic acid (DNA) fragments, the method comprising:

positioning a flow cell containing DNA fragment beads on an xy stage with the flow cell facing an imaging axis of a camera, wherein the xy stage is movable relative to the camera along an x-axis and a y-axis, the y-axis is perpendicular to the x-axis, and the imaging axis is perpendicular to the x-axis and the y-axis;

positioning the xy stage at a first predetermined nominal location along the x-axis and the y-axis with respect to the camera;

illuminating the flow cell from a side of and above the flow cell with a dark field light source to cause a first light to strike the DNA fragment beads at an angle relative to the imaging axis, and reflect off the DNA fragment bead locations;

obtaining a first image of the flow cell;

evaluating the first light reflected from the DNA fragment beads to identify, in the first image, a first reference pattern of bead locations;

moving at least one of the flow cell and the xy stage relative to the camera;

moving the xy stage to reposition the xy stage at a second position substantially at the first predetermined nominal location along the x-axis and the y-axis with respect to the camera;

obtaining a second image of the flow cell;

identifying, in the second image, the first reference pattern of bead locations;

evaluating a first offset, relative to the camera, between the first reference pattern in the first image and the first reference pattern in the second image; and

using the first offset to perform a first correction.

2. The method of claim 1, further comprising using the first offset to perform a first correction comprising at least one of:

moving the xy stage to reposition the flow cell relative to the camera, and

applying a correction factor for reading out intensity values obtained during a fluorescent imaging process.

3. The method of claim 2, wherein moving at least one of the flow cell and the xy stage relative to the camera comprises removing the flow cell from the xy stage and replacing the flow cell on the xy stage.

4. The method of claim 2, wherein moving at least one of the flow cell and the xy stage relative to the camera comprises moving the xy stage in one or both of the x-axis and the y-axis.

5. The method of claim 2, wherein:

the first offset comprises a linear offset between a first position of the flow cell relative to the camera during the step of positioning the xy stage at the first predetermined nominal location, and the second position; and

performing the first correction further comprises correcting for the linear offset by moving the xy stage to place the flow cell at the first position.

6. The method of claim 2, wherein the first offset comprises a linear offset, and applying a correction factor for reading out intensity values obtained during a fluorescent imaging process comprises correcting for the linear offset.

7. The method of claim 2, wherein the first offset comprises an angular offset, and applying a correction factor for reading out intensity values obtained during a fluorescent imaging process comprises correcting for the angular offset.

8. The method of claim 2, further comprising:

positioning the xy stage at a second predetermined nominal location along the x-axis and the y-axis with respect to the camera;

obtaining a third image of the flow cell;

evaluating the first light reflected from the DNA fragment beads to identify, in the third image, a second reference pattern of bead locations;

after the step of moving at least one of the flow cell and the xy stage relative to the camera, moving the xy stage to reposition the xy stage at a fourth position along the x-axis and the y-axis with respect to the camera;

obtaining a fourth image of the flow cell;

identifying, in the fourth image, the second reference pattern of bead locations; and

evaluating a second offset, relative to the camera, between the second reference pattern in the third image and the second reference pattern in the fourth image.

9. The method of claim 8, further comprising using the second offset to perform a second correction comprising at least one of:

moving the xy stage to reposition the flow cell relative to the camera, and

applying a second correction factor for reading out intensity values obtained during the fluorescent imaging process.

10. The method of claim 9, wherein the first correction comprises a linear correction and the second correction comprises an angular correction.

11. The method of claim 8, wherein the first offset comprises a linear offset, and the second offset comprises an angular offset.

12. The method of claim 8, wherein the first offset comprises a first linear offset, the second offset comprises a second linear offset, and the method further comprises:

evaluating the first linear offset and the second linear offset to determine an angular offset.

13. The method of claim 12, further comprising using the angular offset to perform a second correction comprising at least one of:

moving the xy stage to reposition the flow cell relative to the camera, and

applying an angular correction factor for reading out intensity values obtained during the fluorescent imaging process.

14. The method of claim 8, wherein:

the flow cell comprises a plurality of tiles, each tile having a respective reference location;

the first image and the second image are made at a first tile; and

the third image and the fourth image are made at a second tile.

15. The method of claim 14, wherein the second tile is spaced from the first tile.

16. The method of claim 15, wherein the first offset comprises a first linear offset, the second offset comprises a second linear offset, and the method further comprises:

evaluating the first linear offset and the second linear offset to determine an angular offset.

17. The method of claim 16, further comprising using the angular offset to perform a second correction comprising at least one of:

moving the xy stage to reposition the flow cell relative to the camera, and

applying an angular correction factor for reading out intensity values obtained during the fluorescent imaging process.

18. The method of claim 1, further comprising:

obtaining a third image of the flow cell;

evaluating the first light reflected from the DNA fragment beads to identify, in the third image, a second reference pattern of bead locations;

obtaining a fourth image of the flow cell;

identifying, in the fourth image, the second reference pattern of bead locations; and

evaluating a second offset, relative to the camera, between the second reference pattern in the third image and the second reference pattern in the fourth image.

19. The method of claim 18, further comprising evaluating the first offset and the second offset to determine an angular offset.

20. The method of claim 19, further comprising:

using the first offset to perform a first correction comprising at least one of;

moving the xy stage to reposition the flow cell relative to the camera, and

applying a correction factor for reading out intensity values obtained during a fluorescent imaging process; and

using the angular offset to perform a second correction comprising at least one of;

moving the xy stage to reposition the flow cell relative to the camera, and

applying an angular correction factor for reading out intensity values obtained during a fluorescent imaging process.

21. The method of claim 18, further comprising:

using the first offset to perform a first linear correction comprising at least one of;

moving the xy stage to reposition the flow cell relative to the camera, and

applying a linear correction factor for reading out intensity values obtained during a fluorescent imaging process; and

using the second offset to perform an angular correction comprising applying an angular correction factor for reading out intensity values obtained during a fluorescent imaging process.