Method and apparatus for delivery of therapeutic agents

• US 10,252,004 B2
• Filed: 11/14/2016
• Issued: 04/09/2019
• Est. Priority Date: 04/05/2002
• Status: Active Grant

• Current Assignee: Ichor Medical Systems, Inc.
• Original Assignee: Ichor Medical Systems, Inc.
• Inventors: Bernard, Robert M., Hannaman, Andrew W., Bernard, Brady M.
• Primary Examiner(s): Stigell, Theodore
• Application Number:
  US15/351,262
• Publication Number:
  US 20170252515A1
• Time in Patent Office: 876 Days
• CPC Class Codes: A61M 2005/206 With automatic needle insertion
• CPC Class Codes: A61M 5/20 Automatic syringes, e.g. with automatically actuated piston rod, with automatic needle injection, filling automatically A61M5/142 , A61M5/46 take precedence; hypodermic projectiles F42B12/54
• CPC Class Codes: A61M 5/2033 Spring-loaded one-shot injectors with or without automatic needle insertion multishot dosing syringes A61M5/31525, needle insertion only A61M5/3287
• CPC Class Codes: A61M 5/2053 Media being expelled from injector by pressurised fluid or vacuum for infusion A61M5/145, A61M5/155
• CPC Class Codes: A61M 5/30 Syringes for injection by jet action, without needle, e.g. for use with replaceable ampoules or carpules
• CPC Class Codes: A61M 5/326 Fully automatic sleeve extension, i.e. in which triggering of the sleeve does not require a deliberate action by the user
• CPC Class Codes: A61M 5/46 having means for controlling depth of insertion
• CPC Class Codes: A61N 1/30 Apparatus for iontophoresis, i.e. transfer of media in ionic state by an electromotoric force into the body, or cataphoresis
• CPC Class Codes: A61N 1/306 Arrangements where at least part of the apparatus is introduced into the body

Abstract

Methods and apparatus for the reproducible, consistent and efficacious delivery of a therapeutic agent to a patient. The invention comprises means for the controlled administration of the therapeutic agent through an orifice to the patient, a plurality of penetrating electrodes arranged with a predetermined spatial relationship relative to the orifice, and means for generating an electrical signal operatively connected to the electrodes.

Claims

1. A method for intracellular delivery of a therapeutic agent to predetermined tissue site within a patient comprising the steps of:

a. providing an apparatus comprising (i) a main unit comprising at least one injection needle comprising at least one injection orifice, (ii) a sub-assembly unit comprising a plurality of penetrating electrodes, and (iii) an electrical field generator operatively connected to said plurality of penetrating electrodes which provides an electric signal to facilitate the intracellular delivery of said therapeutic agent, and increases intracellular uptake of said therapeutic agent;

wherein a first penetration depth of said plurality of penetrating electrodes is such that said plurality of penetrating electrodes are located within tissue of said patient to encompass said predetermined tissue site, and a second penetration depth of the at least one injection orifice is such that said at least one injection orifice is located within tissue region bounded by said plurality of penetrating electrodes;

wherein the second penetration depth is controllable by a depth controller and controllable independently from and relative to the first penetration depth of the plurality of penetrating electrodes; and

wherein said sub-assembly unit is removably attached to said main unit;

b. inserting said plurality of penetrating electrodes in the tissue of said patient to substantially encompass said predetermined tissue site;

c. contacting said patient with the at least one injection orifice of said at least one injection needle;

d. administering the therapeutic agent through said at least one injection orifice such that the therapeutic agent is distributed within said predetermined tissue site encompassed by said plurality of penetrating electrodes; and

e. applying the electrical signal to at least two of said plurality of penetrating electrodes such that resulting electric field propagation in said predetermined tissue site is of sufficient magnitude and duration to increase the intracellular uptake of said therapeutic agent.

2. The method of claim 1, wherein said apparatus further comprises a template useful to control spatial relationship between said at least one injection orifice and said plurality of penetrating electrodes in the tissue of said patient.

3. The method of claim 1, wherein said apparatus further provides a controlled source of energy that controls rate of agent administration, wherein said controlled source of energy is selected from group consisting of a spring and a compressed gas.

4. The method of claim 1, wherein said electrical signal comprises at least one monopolar direct current pulse, wherein at least one of said monopolar direct current pulse has a duration of 0.1 milliseconds to 100 milliseconds and an amplitude capable of inducing an electrical field of from approximately 50 to approximately 300 V/cm between the at least two of said plurality of penetrating electrodes.

5. The method of claim 1, wherein said main unit is selected from group consisting of a jet injector and an automatic injection device.

6. The method of claim 1, wherein said plurality of penetrating electrodes comprises a conductive, electrochemically stable compound comprising platinum, platinum-iridium, iridium, iridium-oxide, gold, or titanium nitride, or combinations thereof.

7. The method of claim 1, wherein said therapeutic agent is a nucleic acid.

8. The method of claim 1, wherein said predetermined tissue site is located in a skeletal muscle of said patient, wherein the predetermined tissue site comprises healthy cells.

9. A method for intracellular delivery of a therapeutic agent to predetermined tissue site within a patient comprising the steps of:

a. providing an apparatus comprising (i) a main unit comprising at least one injection needle comprising at least one injection orifice, (ii) a sub-assembly unit comprising a plurality of penetrating electrodes, and (iii) an electrical field generator operatively connected to said plurality of penetrating electrodes which provides an electric signal to facilitate the intracellular delivery of said therapeutic agent, and increases the intracellular uptake of said therapeutic agent;

wherein a first tip of said at least one injection needle is proximal to a second tip of said plurality of penetrating electrodes;

wherein a penetration depth of the at least one injection orifice is such that said at least one injection orifice is located within tissue region bound by said plurality of penetrating electrodes; and

wherein said sub-assembly unit is attachable to said main unit;

b. inserting said plurality of penetrating electrodes in tissue of said patient to substantially encompass said predetermined tissue site;

c. contacting said patient with at least one of the at least one injection orifice of the at least one injection needle;

d. administering the therapeutic agent through at least one of said at least one injection orifice such that the agent is distributed within said predetermined tissue site encompassed by said electrodes; and

e. applying the electrical signal to at least two of said plurality of penetrating electrodes such that resulting electric field propagation in said predetermined tissue site is of sufficient magnitude and duration to increase the intracellular uptake of said therapeutic agent.

10. The method of claim 9, wherein said apparatus further comprises a template useful to control spatial relationship between said at least one injection orifice and said plurality of penetrating electrodes in the tissue of said patient.

11. The method of claim 9, wherein said apparatus further provides a controlled source of energy that controls rate of agent administration, wherein said controlled source of energy is selected from group consisting of a spring and a compressed gas.

12. The method of claim 9, wherein said electrical signal comprises at least one monopolar direct current pulse, wherein at least one said monopolar direct current pulse has at least one of:

a duration of 0.1 milliseconds to 100 milliseconds, and an amplitude capable of inducing an electrical field of from approximately 50 to approximately 300 V/cm between the at least two of said plurality of penetrating electrodes.

13. The method of claim 9, wherein said main unit is selected from group consisting of a jet injector and an automatic injection device.

14. The method of claim 9, wherein said plurality of penetrating electrodes comprises a conductive, electrochemically stable compound comprising platinum, platinum-iridium, iridium, iridium-oxide, gold, or titanium nitride, or combinations thereof.

15. The method of claim 9, wherein said therapeutic agent is a nucleic acid.

16. The method of claim 9, wherein said predetermined tissue site is located in a skeletal muscle of said patient, wherein the predetermined tissue site comprises healthy cells.

17. A method for intracellular delivery of a therapeutic agent to predetermined tissue site within a patient comprising the steps of:

a. providing an apparatus comprising (i) a main unit comprising at least one injection needle comprising at least one injection orifice, (ii) a sub-assembly unit comprising a plurality of penetrating electrodes in a retracted state such that the plurality of penetrating electrodes are not visible to the patient, and (iii) an electrical field generator operatively connected to said plurality of penetrating electrodes which provides an electric signal to facilitate the intracellular delivery of said therapeutic agent, and increases intracellular uptake of said therapeutic agent;

wherein said sub-assembly unit is removably attached to said main unit;

b. contacting said patient with the at least one injection orifice of the at least one injection needle wherein a penetration depth of the at least one injection orifice is such that said at least one injection orifice is located within the tissue region bounded by said plurality of penetrating electrodes;

c. administering the therapeutic agent through said at least one injection orifice such that the agent is distributed within said predetermined tissue site encompassed by said plurality of penetrating electrodes; and

d. deploying said plurality of electrodes from their retracted state into tissue of said patient so as to substantially encompass said predetermined tissue site; and

e. applying an electrical signal to at least two of said plurality of penetrating electrodes such that the resulting electric field propagation in said predetermined tissue site is of sufficient magnitude and duration to increase the intracellular uptake of said therapeutic agent.

18. The method of claim 17, wherein said apparatus further comprises a template to control spatial relationship between said at least one injection orifice and said plurality of penetrating electrodes in the tissue of said patient.

19. The method of claim 17, wherein said predetermined tissue site is located in a skeletal muscle of said patient.

20. The method of claim 17, wherein the plurality of penetrating electrodes are deployed after the therapeutic agent is administered through the at least one injection orifice.