Microfluidic devices and arrangements for introducing reagents and biological samples to such devices
First Claim
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1. A microfluidic device for assaying a biological sample, the device comprising:
- a single layer solid support impregnated with the one or more reagents including a weak base, a chelating agent, an anionic surfactant, and/or a chaotropic agent, and wherein the solid support carries both the one or more reagents and a biological sample, a body including a receiving area for receiving the biological sample and one or more reagents carried on the solid support, a receiving chamber at the receiving area, at least one microfluidic port, and a fluid processing area, wherein the receiving chamber is in microfluidic communication with the fluid processing area via said at least one microfluidic port such that the device is configured to assay the biological sample using microfluidics,at least one punch assembly for punching a portion of the solid support and delivering said portion together with the sample and the one or more reagents to the receiving chamber at the receiving area.
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Abstract
A microfluidic device 100 is disclosed comprising a body 112 including a biological sample S and/or reagent R receiving slot 122. The sample and reagent are carried on a solid support 10. The device 100 includes two punch assemblies 130 for removing portions of the solid support 10 and delivering them, together with the sample and/or reagent(s), to a receiving chamber 126 for subsequent processing. The device can be used for various assays including those which require just the addition of water in the reaction chamber with no pre-treatment of sample prior delivery into the chamber.
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Citations
16 Claims
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1. A microfluidic device for assaying a biological sample, the device comprising:
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a single layer solid support impregnated with the one or more reagents including a weak base, a chelating agent, an anionic surfactant, and/or a chaotropic agent, and wherein the solid support carries both the one or more reagents and a biological sample, a body including a receiving area for receiving the biological sample and one or more reagents carried on the solid support, a receiving chamber at the receiving area, at least one microfluidic port, and a fluid processing area, wherein the receiving chamber is in microfluidic communication with the fluid processing area via said at least one microfluidic port such that the device is configured to assay the biological sample using microfluidics, at least one punch assembly for punching a portion of the solid support and delivering said portion together with the sample and the one or more reagents to the receiving chamber at the receiving area. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method of using a microfluidic device to provide an assay, the method comprising the following steps:
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a) providing a microfluidic device comprising a single layer solid support impregnated with the one or more reagents including a weak base, a chelating agent, an anionic surfactant, and/or a chaotropic agent, and wherein the solid support carries both the one or more reagents and a biological sample, a body including a receiving area for receiving the biological sample and one or more reagents carried on the solid support, a receiving chamber at the receiving area, at least one microfluidic port, and a fluid processing area, wherein the receiving chamber is in microfluidic communication with the fluid processing area via said at least one microfluidic port such that the device is configured to assay the biological sample using microfluidics, at least one punch assembly for punching a portion of the solid support and delivering said portion together with the sample and the one or more reagents to the receiving chamber at the receiving area; b) removing the portion of the support which carries at least the portion of the sample; c) delivering the support portion carrying the sample to the receiving chamber; and d) operating the microfluidic device to process said portion according to requirements of the assay. - View Dependent Claims (12, 13, 14, 15, 16)
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Specification