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Automated cell processing methods, modules, instruments, and systems

  • US 10,253,316 B2
  • Filed: 06/30/2018
  • Issued: 04/09/2019
  • Est. Priority Date: 06/30/2017
  • Status: Active Grant
First Claim
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1. An automated stand-alone multi-module cell editing instrument comprising:

  • a housing configured to house all or some of the modules;

    a receptacle configured to receive cells;

    one or more receptacles configured to receive nucleic acids;

    a growth module for growing cells for transformation;

    a transformation module configured to introduce the nucleic acids into the cells;

    a nuclease-directed editing module configured to allow the introduced nucleic acids to edit nucleic acids in the cells;

    a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script; and

    an automated liquid handling system to move liquids from the cell receptacle to the growth module, from the growth module to the transformation module and from the transformation module to the nuclease-directed editing module without user intervention.

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