Sample block apparatus and method for maintaining a microcard on a sample block

• US 10,253,361 B2
• Filed: 08/21/2013
• Issued: 04/09/2019
• Est. Priority Date: 07/30/2002
• Status: Expired due to Term

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Inventors: Fawcett, Adrian
• Primary Examiner(s): Beisner, William H.
• Application Number:
  US13/972,735
• Publication Number:
  US 20130345097A1
• Time in Patent Office: 2,057 Days
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2300/0609 Holders integrated in container to position an object
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/1805 Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 9/523 for multisample carriers, e.g. used for microtitration plates
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: Y10S 435/809 Incubators or racks or holders for culture plates or containers

Abstract

A thermal cycling device for thermally cycling samples of biological material contained in a microcard having a top and bottom surface. The thermal cycling device can include a sample block having an upper surface configured for engaging the bottom surface of a microcard, a vacuum device, and a temperature control system operatively connected with the sample block. The upper surface of the sample block may include a plurality of channels, the channels defining spaces between the sample block and the bottom surface of a microcard that may be positioned thereon. The vacuum device may be in fluid communication with the sample block for drawing gas out of the spaces defined by the channels in the sample block. The vacuum device may be configured for substantially maintaining a vacuum between the sample block and microcard so that a retention force is imparted on the microcard to urge the microcard toward the sample block. Methods of maintaining a microcard on a sample block of a thermal cycling device are also provided.

Claims

1. A biological analysis system, comprising:

a sample block having an upper surface sized and shaped to support a rigid substrate;

a gasket surrounding a region of the upper surface of the sample block, the gasket being positioned between the sample block and the rigid substrate when the rigid substrate is supported over the region of the sample block;

a heater in thermal communication with the sample block;

a controller operatively connected to the heater, the controller programmed to control the heater to raise and lower a temperature of the sample block through at least two repetitive cycles according to a user defined profile;

one or more vacuum ports opening within the region of the upper surface of the sample block; and

a vacuum source in fluid communication with the one or more vacuum ports to draw gas out of a space above the region, thereby creating a vacuum that exerts a force sufficient to retain the substrate in position over the region of the sample block.

2. The system of claim 1, further comprising an optical detection system positioned above the substrate when the substrate is supported over the region of the upper surface of the sample block, and wherein the optical detection system is configured to detect at least one optical signal resulting from at least one biological process occurring in the substrate.

3. The system of claim 1, wherein the controller is configured to provide substantial temperature uniformity across the substrate during at least one biological process occurring in the substrate.

4. The system of claim 1, wherein the region of the upper surface of the sample block comprises a plurality of intersecting grooves.

5. The system of claim 1, wherein the region of the upper surface of the sample block comprises a plurality of substantially parallel grooves.

6. The system of claim 1, wherein the region of the upper surface of the sample block comprises a first plurality of substantially parallel grooves intersecting a second plurality of substantially parallel grooves.

7. The system of claim 1, wherein the region of the upper surface of the sample block comprises a plurality of lateral and longitudinal grooves.

8. The system of claim 1, wherein the region of the upper surface of the sample block comprises a plurality of perpendicular and parallel grooves.

9. The system of claim 1, wherein a sealed volume is created between the upper surface of the region, the gasket, and the substrate when the substrate is supported over the region.

10. A system, comprising:

a rigid substrate having a first surface and a second surface, the rigid substrate being configured to contain at least one DNA sample, and having at least one opening permitting light to pass through;

a sample block having an upper surface sized and shaped to support the substrate;

a gasket surrounding a region of the upper surface of the sample block, wherein a portion of the first surface of the substrate is configured to overlay the gasket when the substrate is supported over the region of the sample block;

a heater in thermal communication with the sample block;

a controller operatively connected to the heater, the controller programmed to control the heater to raise and lower a temperature of the sample block through at least two repetitive cycles according to a user defined profile;

one or more vacuum ports opening within the region of the upper surface of the sample block; and

a vacuum source in fluid communication to create a vacuum within a volume defined between the region and the substrate when the substrate is supported over the region, the vacuum being sufficient to urge the substrate toward the sample block to retain the substrate in position over the region of the sample block while the temperature of the sample block is raised and lowered.

11. The system of claim 10 wherein the region of the upper surface of the sample block comprises at least one of a plurality of channels, recesses, or voids.

12. The system of claim 11, wherein the region of the upper surface of the sample block further comprises a plurality of intersecting grooves.

13. The system of claim 11, wherein the region of the upper surface of the sample block further comprises a plurality of substantially parallel grooves.

14. The system of claim 11, wherein the region of the upper surface of the sample block further comprises a first plurality of substantially parallel grooves and a second plurality of substantially parallel grooves intersecting the first plurality of substantially parallel grooves.

15. The system of claim 11, wherein the region of the upper surface of the sample block further comprises a plurality of lateral and longitudinal grooves.

16. The system of claim 11, wherein the region of the upper surface of the sample block comprises a plurality of perpendicular and parallel grooves.

17. The system of claim 10, further comprising an optical detector, wherein an entire surface area of the second surface of the substrate is optically aligned with the optical detector.