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Diacylgylcerol acyl transferase (DGA1) polynucleotides, and methods of increasing yeast cell lipid production by overexpression of heterologous DGA1

  • US 10,260,052 B2
  • Filed: 02/24/2015
  • Issued: 04/16/2019
  • Est. Priority Date: 02/24/2014
  • Status: Active Grant
First Claim
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1. A method for producing a recombinant Yarrowia cell that has a higher lipid levels than a non-recombinant Yarrowia cell, the method comprising the steps of:

  • a) introducing into a Yarrowia cell a nucleic acid molecule encoding a heterologous polypeptide comprising SEQ ID NO;

    9 or a biologically-active portion thereof; and

    b) cultivating the recombinant Yarrowia cell, wherein the recombinant Yarrowia cell has a higher lipid content than a non-recombinant cell of the same species.

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