Integrated disposable chip cartridge system for mobile multiparameter analyses of chemical and/or biological substances

• US 10,261,041 B2
• Filed: 03/27/2013
• Issued: 04/16/2019
• Est. Priority Date: 03/29/2012
• Status: Active Grant

• Current Assignee: Fraunhofer Gesellschaft Zur Foerderung Der Angewandten Forsching E.V.
• Original Assignee: Fraunhofer Gesellschaft Zur Foerderung Der Angewandten Forsching E.V.
• Inventors: Blohm, Lars, Nebling, Eric, Albers, Joerg, Piechotta, Gundula
• Primary Examiner(s): Mekhlin, Eli S
• Application Number:
  US14/388,702
• Publication Number:
  US 20150060303A1
• Time in Patent Office: 2,211 Days
• Field of Search: None
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/028 Modular arrangements
• CPC Class Codes: B01L 2200/04 Exchange or ejection of cartridges, containers or reservoirs
• CPC Class Codes: B01L 2200/148 Specific details about calibrations
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/0838 Capillaries
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/527 for a plurality of reagents
• CPC Class Codes: G01N 27/28 Electrolytic cell components

Abstract

The invention relates to a measuring chip cartridge module conducting mobile multi-parameter analyzes of chemical and/or biological substances, comprising

• a sample inlet for the extraneous introduction of a sample substance into said measuring chip cartridge module,
• at least one separate reagent inlet for the extraneous introduction of reagents into said measuring chip cartridge module,
• a measuring chip, the surface of which—designed for sample analysis—at least partially restricts an analysis cell with two openings, such that a liquid surrounding said sample substance can pass into the analysis cell through a first opening for the sample analysis, as well as
• a channel system, in which said sample substance can be conveyed from said sample inlet to said analysis cell and said reagents can be conveyed from at least the one reagent inlet to said analysis cell, wherein
• said channel system has no solid reagents, preferably no other substances apart from air and/or inert gas, prior to the introduction of said sample substance via said sample inlet and/or said reagents via at least the one reagent inlet. Furthermore, the invention relates to a system containing a replaceable cartridge module and likewise replaceable reagent storage and processing module, as well as a process for conducting sample analyzes in this system.

Claims

1. Disposable chip cartridge system for conducting mobile multi-parameter analyses of chemical and/or biological substances, comprising:

a) a replaceable measuring chip cartridge module, comprising

sample inlets for the introduction of a sample substance into the measuring chip cartridge module,

at least one separate reagent inlet for the introduction of reagents into said measuring chip cartridge module,

a measuring chip, the surface of which at least partially confines two analysis cells each with two openings, such that a liquid comprising said sample substance can pass into the analysis cells through a first opening for sample analysis, as well as

two separate channel systems, in which said sample substance can be conveyed from said sample inlets to said analysis cells and said reagents can be conveyed from the at least one reagent inlet to said analysis cells, wherein said channel systems do not comprise solid reagents, and no other substances apart from air and/or inert gas, prior to the introduction of said sample substance via said sample inlets and/or said reagents via at least the one reagent inlet;

as well as

b) a replaceable reagent module designed as a uniform block, having two or more reagent cylinders for receiving reagents, wherein each cylinder is designed with a drive system including a drive piston for conveying said sample substance as well as said reagents in the system and a cylinder outlet for the liquid-tight connection to a reagent inlet of the measuring chip cartridge module,

wherein the cylinder outlets of the replaceable reagent module are closed by a membrane, and the respective reagent inlet or inlets of said measuring chip cartridge module include a hollow needle for puncturing said membrane,

wherein said two identical analysis cells on said surface of said measuring chip designed for the analysis of samples correspond to said two separate channel systems, for simultaneously processing the sample substance and reference substance for the calibration of the sample analysis, and

wherein said two separate channel systems are configured to be connected to two different reagent cylinders via the respective hollow needle and wherein the respective drive systems of said two different reagent cylinders include a coupling designed for synchronously operating the two drive systems.

2. System according to claim 1, in which said analysis cells of said measuring chip cartridge module are flow-through cells, such that a liquid comprising said sample substance can pass into said flow-through cells through the first opening and exit said flow-through cells through a second opening.

3. System according to claim 1, wherein two reagent inlets corresponding to each other are provided, one of which is provided for introducing air or buffer to dilute samples and the other is provided for introducing a reference substance.

4. System according to claim 1, in which said measuring chip cartridge module has two or more reagent inlets for the introduction of two or more different reagents into the channel systems.

5. System according to claim 1, in which said channel systems of said measuring chip cartridge module have a rinsing channel for rinsing said sample substance from said sample inlets for conveying to said analysis cells, wherein one end of said rinsing channel is a reagent inlet for receiving a rinsing fluid.

6. System according to claim 1, in which said channel systems of said measuring chip cartridge module have a first dilution and/or waste chamber, which is connected to the said sample inlets for receiving said sample substance and/or to an inlet for ventilating and/or to a reagent inlet for the further conveying of said sample substance to said analysis cells and/or to a second opening of flow-through cells for receiving a used sample substance, in which a liquid comprising said sample substance can pass into said flow-through cells through the first opening and exit said flow-through cells through the second opening.

7. System according to claim 1, in which said channel systems of said measuring chip cartridge module have one or more mixing chambers, in which said sample substance or a reference substance can be mixed with one or more reagents along the way to said analysis cells, and which are connected for this purpose to said sample inlets and/or one or more reagent inlets.

8. System according to claim 1, in which said measuring chip of said measuring chip cartridge module has an electrical connection for connecting to an external device for reading the measurement data.

9. System according to claim 1, comprising a detachable sampling module for collecting said sample substance with a sample outlet, which can be attached to said sample inlets of said measuring chip cartridge module in a liquid-tight manner.

10. System according to claim 1, further comprising a replaceable reagent module having two identical sets of reagent cylinders.

11. System according to claim 1, in which said sample inlets of said measuring chip cartridge module have a capillary for receiving said sample substance as well as an end cap for closing said capillary after taking a sample.

12. System according to claim 11, in which said end cap has an end cap channel that, during a closed condition of the sample inlets, is linked to the capillary in a liquid-tight manner, in order to connect the capillary to said rinsing channel.