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Cartridge for diagnostic testing

  • US 10,261,047 B2
  • Filed: 06/29/2018
  • Issued: 04/16/2019
  • Est. Priority Date: 07/25/2011
  • Status: Active Grant
First Claim
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1. A method of detecting a concentration of Troponin in a whole blood sample, the method comprising:

  • inserting a diagnostic cartridge containing the whole blood sample in a sample chamber into a reader, the diagnostic cartridge having Troponin reporter antibody amended with an enzyme label in the sample chamber and/or in a detection channel; and

    operating the reader tomove the sample out of the sample chamber into the detection channel and across an electrode array in the detection channel, a first electrode of the electrode array having affixed thereon an anti-Troponin capture antibody,move the sample out of the detection channel,move a wash liquid from a first reservoir in the cartridge into the detection channel,move the wash liquid out of the detection channel, andmove a substrate solution out of a second reservoir into the detection channel, the substrate solution comprising a component reactive with the enzyme amended to the reporter antibody; and

    detect a reaction between the Troponin reporter antibody amended with enzyme and the substrate solution to measure the concentration of Troponin in the whole blood sample with a limit of detection of 3 pg/mL.

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