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Systems and methods for genetic and biological analysis

  • US 10,266,892 B2
  • Filed: 06/13/2018
  • Issued: 04/23/2019
  • Est. Priority Date: 05/27/2011
  • Status: Active Grant
First Claim
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1. A system for nucleic acid sequencing, comprising:

  • an array comprising a plurality of features in optical communication with a plurality of optical sensors, wherein said array is integrated with said plurality of optical sensors, wherein, during use, a feature of said plurality of features retains or immobilizes a plurality of nucleic acid molecules having sequences that are substantially identical to a nucleic acid sequence of a template nucleic acid molecule, and wherein an optical sensor of said plurality of optical sensors is in optical communication with said feature; and

    one or more computer processors operatively coupled to said array, wherein said one or more computer processors are programmed to (i) bring said plurality of nucleic acid molecules in contact with nucleic acid bases in the presence of polymerizing enzymes under conditions sufficient to incorporate said nucleic acid bases into growing strands complementary to said plurality of nucleic acid molecules, wherein said plurality of nucleic acid molecules is brought in contact with said nucleic acid bases without applying an electric field to confine or isolate said nucleic acid bases within said feature, (ii) use said optical sensor to detect one or more signals indicative of said nucleic acid bases incorporated into said growing strands, and (iii) use said one or more signals detected in (ii) to identify said nucleic acid bases, to thereby generate a sequence corresponding to said nucleic acid sequence of said template nucleic acid molecule.

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