Substance detection device and method
First Claim
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1. A method of detecting an analyte in a medium, the method comprising:
- (a) contacting the medium with an analyte detection device, the analyte detection device comprising;
a first electrode that contacts with the medium, the first electrode comprising an electrogenic bacterium genetically modified to be selectively reactive with a substance to generate electrons, and wherein the substance is the analyte or is produced as a result of the presence of the analyte in the medium;
a second electrode that is an electron acceptor of electrons generated at the first electrode, wherein electrons are received from the first electrode to provide power to the analyte detection device; and
a signal generator that produces a signal from the electrons;
(b) measuring the amount of signal from the signal generator; and
(c) relating the amount of signal to one or both of the presence and the amount of the analyte in the medium,wherein the analyte acts to cleave a substance precursor in the medium to release the substance.
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Abstract
Devices and methods are employed to detect substances in a medium. The device comprises an electrogenic bacterium that selectively interacts with a substance to produce electrons. A portion of the electrons provides power to the device and a portion of the electrons generates a signal as an indication of the presence of a substance in the medium. The method comprises contacting the electrogenic bacterium of the device with a medium suspected of containing the substance and measuring the signal generated by the electrons.
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Citations
12 Claims
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1. A method of detecting an analyte in a medium, the method comprising:
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(a) contacting the medium with an analyte detection device, the analyte detection device comprising; a first electrode that contacts with the medium, the first electrode comprising an electrogenic bacterium genetically modified to be selectively reactive with a substance to generate electrons, and wherein the substance is the analyte or is produced as a result of the presence of the analyte in the medium; a second electrode that is an electron acceptor of electrons generated at the first electrode, wherein electrons are received from the first electrode to provide power to the analyte detection device; and a signal generator that produces a signal from the electrons; (b) measuring the amount of signal from the signal generator; and (c) relating the amount of signal to one or both of the presence and the amount of the analyte in the medium, wherein the analyte acts to cleave a substance precursor in the medium to release the substance. - View Dependent Claims (2, 3, 4)
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5. A method of detecting an analyte in a medium, the method comprising:
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(a) contacting the medium with an electrogenic bacterium that selectively interacts with the analyte or a substance produced as a result of the presence of the analyte in the medium to produce electrons wherein a portion of the electrons provide power and wherein a portion of the electrons generate a signal as an indication of the presence of the substance in the medium; and (b) measuring the signal, wherein the analyte acts to cleave a substance precursor in the medium to release the substance. - View Dependent Claims (6, 7, 8, 9, 10, 11, 12)
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Specification