Medical fluid, a method of treatment and use of the fluid

• US 10,271,540 B2
• Filed: 08/05/2016
• Issued: 04/30/2019
• Est. Priority Date: 05/14/2010
• Status: Active Grant

• Current Assignee: Xvivo Perfusion AB
• Original Assignee: Xvivo Perfusion AB
• Inventors: Steen, Stig
• Primary Examiner(s): Cordas, Emily A
• Application Number:
  US15/229,356
• Publication Number:
  US 20160338343A1
• Time in Patent Office: 998 Days
• Field of Search: None
• CPC Class Codes: A01N 1/0226 Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients

Abstract

A medical fluid for a harvested organ, tissue or part thereof, for evaluation and/or preservation. The fluid includes cocaine or a stimulating analog thereof; noradrenaline; and/or adrenaline. In addition, the fluid includes an oncotic agent, such as dextran; hormones, such as thyroxin; triiodotyronine; cortisone, insulin; and electrolytes and optionally nutrients in substantially physiological concentrations in a physiologically acceptable medium. In addition, the medical fluid further includes albumin in a concentration not exceeding 5.0%, and an oxygen carrier, such as erythrocytes. Further components may be dopamine; hydrocortisone; methylprednisolone; and a vasopressor agent, such as desmopressin. The cocaine; adrenaline; and noradrenaline are present in concentrations of each about 0.010 μM to 0.100 μM, for example in a ratio of 1:1:1.

Claims

1. A method for preservation and/or evaluation of a harvested organ comprising:

circulating a medical fluid in the vascular system of the harvested organ, wherein;

the medical fluid comprises;

cocaine or a cocaine analogue with stimulating effect;

adrenaline; and

noradrenaline; and

the harvested organ is at least partly denervated,

wherein the cocaine or the cocaine analogue is present in the medical fluid at a concentration of 0.010 μ

M to 0.100 μ

M.

2. The method according to claim 1, wherein the medical fluid further comprises:

an oncotic agent; and

electrolytes in physiological concentrations in a physiologically acceptable medium.

3. The method according to claim 1, wherein the adrenaline and the noradrenaline are present in the medical fluid at concentrations that are subphysiological.

4. The method according to claim 1, wherein:

the adrenaline is present in the medical fluid at a concentration of 0.010 μ

M to 0.100 μ

M; and

the noradrenaline is present in the medical fluid at a concentration of 0.010 μ

M to 0.100 μ

M.

5. The method according to claim 1, wherein the cocaine or the cocaine analogue, the adrenaline, and the noradrenaline are present in the medical fluid at concentrations that are equimolar.

6. The method according to claim 1, wherein the harvested organ comprises a lung, lungs, or a heart.

7. The method according to claim 1, wherein:

the medical fluid further comprises;

an oncotic agent; and

electrolytes in physiological concentrations in a physiologically acceptable medium; and

the adrenaline and the noradrenaline are present in the medical fluid at concentrations that are subphysiological.

8. The method according to claim 1, wherein:

the medical fluid further comprises;

an oncotic agent; and

electrolytes in physiological concentrations in a physiologically acceptable medium;

the adrenaline is present in the medical fluid at a concentration of 0.010 μ

M to 0.100 μ

M; and

the noradrenaline is present in the medical fluid at a concentration of 0.010 μ

M to 0.100 μ

M.

9. The method according to claim 8, wherein the harvested organ comprises a lung, lungs, or a heart.

10. The method according to claim 1, wherein:

the medical fluid further comprises;

an oncotic agent; and

electrolytes in physiological concentrations in a physiologically acceptable medium; and

the cocaine or the cocaine analogue, the adrenaline, and the noradrenaline are present in the medical fluid at concentrations that are equimolar.

11. The method according to claim 1, wherein the medical fluid comprises the cocaine.

12. The method according to claim 11, wherein the medical fluid further comprises:

an oncotic agent; and

electrolytes in physiological concentrations in a physiologically acceptable medium.

13. The method according to claim 11, wherein the adrenaline and the noradrenaline are present in the medical fluid at concentrations that are subphysiological.

14. The method according to claim 11, wherein:

the adrenaline is present in the medical fluid at a concentration of 0.010 μ

M to 0.100 μ

M; and

the noradrenaline is present in the medical fluid at a concentration of 0.010 μ

M to 0.100 μ

M.

15. The method according to claim 11, wherein the cocaine, the adrenaline, and the noradrenaline are present in the medical fluid at concentrations that are equimolar.

16. The method according to claim 11, wherein the harvested organ comprises a lung, lungs, or a heart.