Methods of producing antibodies specific for p95

• US 10,273,308 B2
• Filed: 06/12/2015
• Issued: 04/30/2019
• Est. Priority Date: 12/01/2008
• Status: Active Grant

• Current Assignee: Laboratory Corporation Of America Holdings
• Original Assignee: Laboratory Corporation Of America Holdings
• Inventors: Sperinde, Jeff, Winslow, John William, Jin, Xueguang, Wallweber, Gerald J.
• Primary Examiner(s): Yao, Lei
• Application Number:
  US14/737,742
• Publication Number:
  US 20150376293A1
• Time in Patent Office: 1,418 Days
• Field of Search: None
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: C07K 16/32 against translation products of oncogenes
• CPC Class Codes: C07K 2317/30 characterized by aspects of specificity or valency
• CPC Class Codes: C07K 2317/34 Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
• CPC Class Codes: C07K 2317/73 Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
• CPC Class Codes: C12Q 1/6886 for cancer immunoassay for cancer G01N33/574
• CPC Class Codes: C12Q 2600/158 Expression markers
• CPC Class Codes: G01N 2333/71 for growth factors; for growth regulators
• CPC Class Codes: G01N 2800/52 Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
• CPC Class Codes: G01N 33/5005 involving human or animal cells immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025
• CPC Class Codes: G01N 33/57415 of breast
• CPC Class Codes: G01N 33/57484 involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
• CPC Class Codes: G01N 33/6893 related to diseases not provided for elsewhere
• CPC Class Codes: G01N 33/74 involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors

Abstract

The disclosure provides methods of making antibodies specific for p95.

Claims

1. A method of producing antibodies specific for a p95Her-2 protein fragment having a first amino acid corresponding to methionine 611 of Her-2 protein comprising growing a hybridoma cell line having ATCC accession number PTA-9738 (p95.D3.4), PTA-9739 (p95.D8.2), or PTA-9740 (p95.D9.1) in cell culture medium thereby producing said antibodies.

2. The method of claim 1, wherein said antibodies are purified from said cell culture medium.

3. The method of claim 1, wherein the antibodies are purified by one or more of dialysis, affinity chromatography, gel electrophoresis, protein A-agarose chromatography, or protein L-agarose chromatography.