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Specification of functional cranial placode derivatives from human pluripotent stem cells

  • US 10,273,452 B2
  • Filed: 05/19/2016
  • Issued: 04/30/2019
  • Est. Priority Date: 11/21/2013
  • Status: Active Grant
First Claim
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1. A method for inducing differentiation of cells comprisinga) contacting a plurality of starting cells with an inhibitor of Small Mothers Against Decapentaplegic (SMAD) protein signaling (“

  • the SMAD inhibitor”

    ), wherein the starting cells are selected from the group consisting of multipotent cells, pluripotent cells, and a combination thereof; and

    b) contacting the cells with a bone morphogenetic protein (BMP);

    c) contacting the cells with a compound selected from the group consisting of BRL-54443, parthenolide, phenanthroline, and combinations thereof; and

    wherein the cells are contacted with the SMAD inhibitor and the BMP in an amount effective to induce detectable expression of SIX1 and PAX6 in the plurality of cells.

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