Real-time redox sequencing methods
First Claim
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1. A method for nucleic acid sequencing comprising:
- providing a substrate comprising an oxidizing nanoscale electrode and a reducing nanoscale electrode, the substrate comprising a polymerase enzyme complex comprising a polymerase enzyme and a template nucleic acid, the complex attached to one of the nanoscale redox electrodes or to the substrate proximate to the electrodes;
exposing the polymerase to a plurality of types of nucleotide analogs each comprising a different redox label attached to the phosphate portion of the nucleotide analog through a linker of a length such that the redox label can come into contact with the oxidizing nanoscale electrode and the reducing nanoscale electrode when the nucleotide analog is held in the polymerase enzyme active site under conditions whereby polymerase mediated nucleic acid synthesis occurs, resulting in the growth of a nascent nucleic acid strand;
applying voltages over time at the oxidizing nanoscale electrode and reducing nanoscale electrode, whereby when a nucleotide analog resides in the active site of the enzyme, a redox label is oxidized by the oxidizing nanoscale electrode, and reduced by the reducing nanoscale electrode;
monitoring the current at the oxidizing nanoscale electrode and at the reducing nanoscale electrode over time, whereby the current from multiple oxidations and reductions of a redox label at the electrodes indicates an incorporation event for a nucleotide analog having a specific redox label; and
using the monitored current at the electrodes over time to determine a sequence of the template nucleic acid.
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Abstract
Real time redox sequencing methods, devices, and systems are described. Arrays of redox devices comprising one or two electrodes are used to provide sequence information about a template nucleic acid in a polymerase-template complex bound proximate to the electrode(s). A sequencing reaction mixture comprising nucleotide analogs comprising redox labels is introduced to the array of redox devices under conditions of polymerase mediated nucleic acid synthesis. The time sequence of incorporation of nucleotide analogs is determined by electrochemically identifying the redox labels of the nucleotide analogs that are incorporated into the growing strand.
76 Citations
17 Claims
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1. A method for nucleic acid sequencing comprising:
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providing a substrate comprising an oxidizing nanoscale electrode and a reducing nanoscale electrode, the substrate comprising a polymerase enzyme complex comprising a polymerase enzyme and a template nucleic acid, the complex attached to one of the nanoscale redox electrodes or to the substrate proximate to the electrodes; exposing the polymerase to a plurality of types of nucleotide analogs each comprising a different redox label attached to the phosphate portion of the nucleotide analog through a linker of a length such that the redox label can come into contact with the oxidizing nanoscale electrode and the reducing nanoscale electrode when the nucleotide analog is held in the polymerase enzyme active site under conditions whereby polymerase mediated nucleic acid synthesis occurs, resulting in the growth of a nascent nucleic acid strand; applying voltages over time at the oxidizing nanoscale electrode and reducing nanoscale electrode, whereby when a nucleotide analog resides in the active site of the enzyme, a redox label is oxidized by the oxidizing nanoscale electrode, and reduced by the reducing nanoscale electrode; monitoring the current at the oxidizing nanoscale electrode and at the reducing nanoscale electrode over time, whereby the current from multiple oxidations and reductions of a redox label at the electrodes indicates an incorporation event for a nucleotide analog having a specific redox label; and using the monitored current at the electrodes over time to determine a sequence of the template nucleic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method for nucleic acid sequencing comprising:
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providing a substrate comprising a nanoscale redox electrode, the substrate comprising a polymerase enzyme complex comprising a polymerase enzyme and a template nucleic acid, the complex attached to the nanoscale electrode or to the substrate proximate to the nanoscale electrode; exposing the polymerase to a plurality of types of nucleotide analogs each comprising a different redox label attached to the phosphate portion of the nucleotide analog through a linker of a length such that the redox label can come into contact with the nanoscale redox electrode when the nucleotide analog is held in the polymerase enzyme active site under conditions whereby polymerase mediated nucleic acid synthesis occurs, resulting in the growth of a nascent nucleic acid strand;
wherein the nanoscale electrode is repeatedly brought to a plurality of voltage levels comprising at least one oxidizing voltage and at least one reducing voltage;applying voltages over time to the nanoscale redox electrode, whereby when a nucleotide analog resides in the active site of the enzyme, the redox label is oxidized and reduced by the nanoscale electrode; monitoring the current at nanoscale electrode at the plurality of voltages over time, whereby the current from multiple oxidations and reductions of a redox label at the electrode indicates an incorporation event for a nucleotide analog having a specific redox label; and using the monitored current at the electrode over time to determine a sequence of the template nucleic acid. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17)
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Specification