Device and method of measuring animal health

• US 10,274,433 B1
• Filed: 09/30/2015
• Issued: 04/30/2019
• Est. Priority Date: 09/30/2015
• Status: Active Grant

• Current Assignee: Recursion Pharmaceuticals, Inc.
• Original Assignee: Vium, Inc. (Recursion Pharmaceuticals, Inc.)
• Inventors: Betts-Lacroix, Jonathan, Schaevitz, Laura R., Heath, Kyle Howard
• Primary Examiner(s): Siefke, Samuel P
• Application Number:
  US14/872,060
• Time in Patent Office: 1,308 Days
• CPC Class Codes: A01K 1/031 Cages for laboratory animals; Cages for measuring metabolism of animals
• CPC Class Codes: A01K 23/00 Manure or urine pouches
• CPC Class Codes: A61D 19/00 Instruments or methods for reproduction or fertilisation
• CPC Class Codes: G01N 2021/752 Devices comprising reaction zones
• CPC Class Codes: G01N 2021/7759 Dipstick; Test strip
• CPC Class Codes: G01N 2021/7793 Sensor comprising plural indicators
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

A test pad comprising multiple test patches and urine detection patches in an array is described. The test pad is suitable for automated urine analysis of multiple animals in a cage. Urine detection patches may be arranged in a BAYER pattern or as rings. Multiple layers, including a substrate, a wicking layer, a test patch layer, and optional protection layer serve to manage puddles of urine. Various isolation elements are described that keep urine puddles from spreading. Color reference spots on the pad are used for dynamic color determination of test patches. Urine detection patches start a read clock and identify which test patches are valid. Elements to hold and align the test pad are described. Methods of detection and reading are described.

Claims

1. A device for measuring attributes of urine comprising:

a pad comprising a test patch layer comprising;

a plurality of different urine test patches, each different urine test patch comprising one or more respective urine test chemicals that change color responsive to a respective urine attribute;

a plurality of urine detection patches, wherein the urine detection patches are different from any urine test patch, and wherein each urine detection patch comprises one or more chemicals that change color responsive to presence of urine;

wherein each different urine test patch is at least partially surrounded by one or more of the urine detection patches;

the urine detection patches and urine test patches are arranged in a BAYER pattern, wherein the urine detection patches are arranged in a checkerboard pattern within the BAYER pattern and the urine test patches are distributed among the remaining location in the BAYER pattern;

a wicking layer above the test patch layer;

a transparent protection layer with holes, above the wicking layer;

a non-porous substrate below the test patch layer;

wherein the urine detection patches and the urine test patches are on the test patch layer.

3. The device of claim 1 wherein:

a color of each detection patch and a color of each test patch layer are viewable through the wicking layer.

4. The device of claim 1 further comprising:

at least one scent region comprising a chemical scent attractive to a single sex of a rodent.

5. The device of claim 1 further comprising:

at least one scent region comprising a chemical scent attractive to a single sex of a rodent.

6. The device of claim 1 further comprising:

well boundaries in the substrate layer comprising previously melted portions of the substrate layer;

wherein wells are defined by the well boundaries;

and wherein the wells are fluidly isolated from each other;

and wherein each well comprises at least one urine detection patch and at least one urine test patch.

7. The device of claim 1 wherein:

portions of the wicking layer protrude into the holes in the transparent protection layer.

2. A device for measuring attributes of urine comprising:

a pad comprising a test patch layer comprising;

a plurality of different urine test patches, each different urine test patch comprising one or more respective urine test chemicals that change color responsive to a respective urine attribute;

a plurality of urine detection patches, wherein the urine detection patches are different from any urine test patch, and wherein each urine detection patch comprises one or more chemicals that change color responsive to presence of urine;

wherein each different urine test patch is at least partially surrounded by one or more of the urine detection patches;

the urine detection patches and urine test patches are arranged in a BAYER pattern, wherein the urine detection patches are arranged in a checkerboard pattern within the BAYER pattern and the urine test patches are distributed among the remaining location in the BAYER pattern;

a protection layer above the test patch layer, transparent or translucent;

comprising holes adapted to pass urine from above the top layer into the test patch layer;

a urine impenetrable substrate layer below the test patch layer.