Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
First Claim
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1. A method of analyzing at least one macromolecule, comprising the steps of:
- transporting at least one macromolecule in a fluid sample into a nanochannel, such that the at least one macromolecule is in a fully elongated state when completely inside the nanochannel;
maintaining the at least one macromolecule in the fully elongated state within the nanochannel without application of a shear force;
detecting at least one signal transmitted from the at least one fully elongated macromolecule within the nanochannel; and
correlating the detected signal to at least one property of the at least one macromolecule.
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Abstract
Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.
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33 Claims
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1. A method of analyzing at least one macromolecule, comprising the steps of:
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transporting at least one macromolecule in a fluid sample into a nanochannel, such that the at least one macromolecule is in a fully elongated state when completely inside the nanochannel; maintaining the at least one macromolecule in the fully elongated state within the nanochannel without application of a shear force; detecting at least one signal transmitted from the at least one fully elongated macromolecule within the nanochannel; and correlating the detected signal to at least one property of the at least one macromolecule. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A method of maintaining at least one macromolecule in fully elongated state, comprising the steps of:
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transporting at least one macromolecule in a fluid sample into a nanochannel, such that the at least one macromolecule is in a fully elongated state when completely inside the nanochannel; and maintaining the at least one macromolecule in fully elongated state within the nanochannel without application of a shear force. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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Specification