Preparations containing hepatocyte growth factor and hyaluronic acid, and methods of making and using same

• US 10,279,075 B2
• Filed: 10/28/2016
• Issued: 05/07/2019
• Est. Priority Date: 03/15/2013
• Status: Active Grant

• Current Assignee: Prime Merger Sub LLC (Alight, Inc.)
• Original Assignee: NuTech Medical Incorporated (Organogenesis Holdings, Inc.)
• Inventors: Walthall, Jr., Howard P.
• Primary Examiner(s): Allen, Marianne P
• Application Number:
  US15/337,427
• Publication Number:
  US 20170043054A1
• Time in Patent Office: 921 Days
• Field of Search: None
• CPC Class Codes: A61K 2800/91 Injection
• CPC Class Codes: A61K 38/1833 Hepatocyte growth factor; Scatter factor; Tumor cytotoxic factor II
• CPC Class Codes: A61K 8/64 Proteins; Peptides; Derivatives or degradation products thereof
• CPC Class Codes: A61K 8/735 Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
• CPC Class Codes: A61L 2300/414 Growth factors
• CPC Class Codes: A61L 2400/06 Flowable or injectable implant compositions
• CPC Class Codes: A61L 2430/02 for reconstruction of bones; weight-bearing implants
• CPC Class Codes: A61L 2430/06 for cartilage reconstruction, e.g. meniscus
• CPC Class Codes: A61L 2430/10 for reconstruction of tendons or ligaments
• CPC Class Codes: A61L 2430/24 for joint reconstruction
• CPC Class Codes: A61L 2430/32 for nerve reconstruction
• CPC Class Codes: A61L 2430/34 for soft tissue reconstruction
• CPC Class Codes: A61L 2430/38 for reconstruction of the spine, vertebrae or intervertebral discs
• CPC Class Codes: A61L 27/20 Polysaccharides
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61L 27/56 Porous materials, e.g. foams or sponges
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61Q 19/08 Anti-ageing preparations
• CPC Class Codes: C08L 5/08 Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof

Abstract

Preparations containing hepatocyte growth factor (HGF) and hyaluronic acid (HA) and methods of making and using same. The HGF and HA preparations can be prepared together in solution as an injectable fluid without gelatinization, or impregnated within a porous hydrophilic matrix material with, or without, cross-linking of the HA with the matrix material. The preparations can be used as a dermal filler or to generate and promote healing of cartilage, vertebral discs, connective tissues such as tendons and ligaments and bone in vivo.

Claims

1. A composition for regenerating damaged or defective tissue comprising:

a porous matrix,

hyaluronic acid (HA) cross-linked in situ within the porous matrix in the presence of cross-linking heavy chains of inter-alpha inhibitor and Tumor Necrosis Factor Stimulating Gene-6, and hepatocyte growth factor (HGF),

wherein there is limited or no chemical cross-linking of the HA with the porous matrix.

2. The composition of claim 1 wherein the HA is cross-linked in situ within the porous matrix by soaking the porous matrix with the HA, the cross-linking heavy chains of inter-alpha inhibitor and Tumor Necrosis Factor Stimulating Gene 6.

3. The composition of claim 1 wherein the HGF is added during cross-linking of HA in situ within the porous matrix.

4. The composition of claim 1, wherein the HGF is added by soaking the porous matrix in HGF after cross-linking of HA in situ within the porous matrix.